胰岛素治疗大鼠重症急性胰腺炎的机制探讨

目的探讨胰岛素在大鼠重症急性胰腺炎发病机制中的保护作用。方法 60只雌性SD大鼠随机分为正常对照组、重症胰腺炎组、胰岛素干预组,各组按照24 h、48 h组各分配10只。采用5%牛磺胆酸钠逆行胰胆管注射的方法,复制大鼠重症急性胰腺炎损伤模型。胰岛素干预组给与1IU/kg的胰岛素,1日3次腹腔灌注。用全自动生化分析仪测定大鼠血清淀粉酶,用硫代巴比妥法测定丙二醛(MDA)含量,黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活力;应用血糖仪(美国强生血糖仪)监测血糖。结果 SAP造模后各组大鼠血清淀粉酶,血糖值,MDA含量逐渐升高,SOD活力降低;胰岛素干预后各组大鼠血清淀粉酶,血糖值,MDA含量逐渐降低,SOD活力增加。结论胰岛素可能通过对抗大鼠重症急性胰腺炎发病过程的脂质过氧化起保护作用。

Mechanism of Insulin Therapy on Rats With Severe Acute Pancreatitis.

Objective To observe the protective effect of insulin in rats with severe acute pancreatitis. Methods Sixty female SD rats were randomly divided into normal control group, severe pancreatitis group, the insulin intervention group. Each group in accordance with 24 hours, 48 hours has 10 rats. 5% sodium taurocholate was retrograde injected into the biliopancreatic duct of the rats to induce SAP. Insulin intervention groups were given 1 IU/kg insulin by intraperitoneal peffusion, three times a day. Serum amylase was detected by automatic biochemical analyzer. The MDA was measured by thiobarbituric content, and the determination of SOD activity was xanfline oxidase. Blood glucose was detected by using the blood glucose monitor of Johnson ＆ Johnson. Results The serum amylase, blood glucose levels, MDA content of SAP groups increased, with the SOD activity reduced, while the serum amylase, blood glucose levels, MDA content of insulin intervention groups decreased, with the SOD activity increased. Conclusion Insulin may protect rats in severe acute pancreatitis through fighting against the lipid peroxidadon process.