| 应用原位杂交技术检测乳腺癌组织中microRNA的表达 |
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| 引用本文： | 崔秀英,吴建南,于风燕. 应用原位杂交技术检测乳腺癌组织中microRNA的表达[J]. 岭南现代临床外科, 2009, 9(2): 120-123 |
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| 作者姓名： | 崔秀英 吴建南 于风燕 |
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| 作者单位： | 510120,中山大学附属第二医院乳腺外科;510120,中山大学附属第二医院乳腺外科;510120,中山大学附属第二医院乳腺外科 |
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| 基金项目： | 广东省医学科技术研究基金 |
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| 摘 要： | 目的检测乳腺癌组织中let-7 microRNA的表达状况。方法采用地高辛配基标记探针的非同位素原位杂交技术检测35例乳腺癌组织石蜡切片。结果在35例标本中,25例乳腺癌组织的石蜡切片let-7 microRNA阳性表达,蓝紫色的阳性染色颗粒位于癌细胞的胞浆,背景清晰。结论建立了一种检测let-7 microRNA的方法,对乳腺癌组织进行检测,为以后研究乳腺癌致病机制,诊断和靶向治疗乳腺癌打下实验基础。
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| 关 键 词： | 乳腺癌 原位杂交 let-7 microRNA |
Expression of microRNA in human breast cancer detected by in situ hybridization |
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| Cui Xiuying,Wu Jiannan,Yu Fengyan. Expression of microRNA in human breast cancer detected by in situ hybridization[J]. Lingnan Modern Clinics in Surgery, 2009, 9(2): 120-123 |
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| Authors: | Cui Xiuying Wu Jiannan Yu Fengyan |
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| Affiliation: | Cui Xiuying,Wu Jiannan,Yu Fengyan.Breast Tumor Centre,The Second Affiliated Hospital,Sun Yat-sen University,Guang zhou 510120 |
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| Abstract: | Objective To detect the expression of let-7 microRNA in human breast cancer.Methods In situ hybridization(ISH)of formalin-fixed,paraffin-embedded(FFPE) archival tissues sections of 35 human breast cancer have been done using digoxigenin labeled let-7 probe to identify the expression of let-7.Results ISH with LNA probes against let-7 microRNAwere performed in 35 human breast cancer.Let-7 had particularly high levels of expression in 25 samples.Conclusion This suggests that the technique of ISH is a specific,... |
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| Keywords: | let-7 microRNA |
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