丹皮酚对大鼠脑缺血再灌注损伤后TLR4及神经细胞凋亡的影响

 目的 观察丹皮酚对大鼠局灶性脑缺血再灌注损伤后 Toll样受体4（TLR4）和神经细胞凋亡的影响，并探讨其作用机制。方法 大鼠随机分为假手术组、脑缺血再灌注模型组、尼莫地平组（0.4 mg·kg^-1）、丹皮酚组（15、30 mg·kg^-1）。采用线栓法阻塞大脑中动脉建立大鼠局灶性脑缺血再灌注模型。通过 HE染色观察大鼠大脑皮层区细胞损伤程度，免疫组化法检测缺血侧脑组织 TLR4表达的变化，酶联免疫吸附法测定血清白细胞介素6（IL-6）含量，流式细胞仪检测神经细胞凋亡。结果 丹皮酚可明显改善大鼠皮层区细胞损伤，减少 TLR4的表达、降低血清中 IL-6含量，减轻神经细胞凋亡率。结论 丹皮酚能减轻缺血再灌注脑组织损伤程度、降低神经细胞凋亡率，机制可能与抑制脑组织内 TLR4表达有关。

Effect of Paeonol on TLR4 and Apoptosis in Rats with Focal Cerebral Ischemia-Reperfusion Injury

OBJECTIVE To investigate the effects of paeonol on Toll-like receptor (TLR4) and neuron apoptosis after focal cerebral ischemia-reperfusion injury and its possible mechanism in rats. METHODS 100 Rats were randomly divided into sham group， ischemia-reperfusion group， nimodipine group， and paeonol groups (15 and 30 mg·kg^-1). Focal cerebral ischemic model was established by middle cerebral artery occlusion (MCAO). The morphological changes of cerebral cortical neurons through HE staining were observed. The content of IL-6 in serum was measured by ELISA. The apoptosis was detected by flow cytometry. RESULTS Paeonol decreased neuronal edema and neuronal apoptosis of cortex， reduced the content of IL-6 in serum and TLR4 in brain. CONCLUSION Paeonol could relieve brain damage induced by focal cerebral ischemia-reperfusion， and decrease neuronal apoptosis. The effects may be related to the decrease of TLR4 content in brain.