噻唑蓝法测定抗肿瘤复方制剂仙慈丹的体外抗肿瘤活性

 目的 分离筛选出抗肿瘤复方制剂仙慈丹（XCD）的抗肿瘤活性部位。方法 采用大孔树脂柱色谱法对抗肿瘤复方制剂XCD水提取液进行分段富集,运用噻唑蓝（MTT）比色法对分段富集得到的部位进行体外抗肿瘤活性测定。结果 抗肿瘤复方制剂XCD水提取液过AB-8型树脂纯化得到的P2（体积分数20%乙醇洗脱液）、P3（体积分数40%乙醇洗脱液）、P4（体积分数60%乙醇洗脱液）、P5（体积分数80%乙醇洗脱液）对人肝癌HepG2细胞表现出较强的抑制作用。结论 XCD水提液过大孔树脂后乙醇洗脱部位是XCD制剂的抗肿瘤活性部位。

Determination of Anti-Tumor Activity of Compound Preparation Xiancidan in Viro by MTT Method

OBJECTIVE To separate and select anti-tumor activity site of the anti-tumor compound preparation Xiancidan（XCD）. METHODS The means of macroporous resin column chromatography(AB-8) was used to separate the water extract from Chinese drug XCD. The anti-tumor activities of the different extracts in vitro were analyzed with MTT method. RESULTS P2(20% ethanol elution), P3（40% ethanol elution ）,P4(60% ethanol elution) and P5(80% ethanol elution) strongly inhibited cell proliferation of HepG2. CONCLUSION Ethanol elution position is the anti-tumor extract from XCD.