子宫内膜癌胰岛素样生长因子2的表达和印迹状态的改变

Expression level and genomic imprinting status of insulin-like growth factor 2 in endometrial carcinoma

Objective Gene imprinting is a new focus in cancer research,and insulin-like growth factor 2(IGF2) is one of the earliest endogenous imprinting genes ever found.The relationship between cancer and IGF2 has drawn special attention from the medical world.The purpose of this study was to investigate the association of the expression level of IGF2 with the development of endometrial carcinoma.Methods We determined the expression of IGF2 in 37 samples of endometrial carcinoma,25 samples of paracancer tissue and 20 samples of normal tissue by RT-PCR semi-quantitative analysis.Based on the polymorphism of the Apa I site in exon 9 of IGF2,we examined the genomic imprinting status of IGF2 by PCR-RFLP and RT-PCR-RFLP.Results The average expression level of IGF2 was significantly higher in endometrial carcinoma than in paracancer and normal tissues,but with no significant difference between the latter two.The loss of imprinting(LOI) of IGF2 was found in the samples,but with no significant differences among the three groups.Conclusion The increased expression of IGF2 may be associated with the pathogenesis of endometrial carcinoma.But whether the LOI of IGF2 plays a role in endometrial carcinoma deserves further exploration.