金纳米花粒子对体外人成纤维细胞的毒性作用

目的：探讨具有近红外吸收功能的金纳米花粒子对人成纤维细胞的体外细胞毒性作用,为其在生物医学领域的应用提供依据。方法：分离培养人成纤维细胞，采用含不同浓度金纳米花粒子（1.875、3.750、7.500、15.000、30.000 mg·L-^1）的含金培养液进行培养，以不加金纳米粒子组为空白对照组，应用MTT比色法检测其细胞毒效应，倒置
显微镜观察细胞形态。结果：培养液中加入不同浓度金纳米花粒子后，各组细胞相对增殖率(RGR)分别为92.245%、88.980%、86.939%、75.782%和71.756%，加入30.000 mg·L^-1金纳米花组，其RGR与空白对照组比较差异有统计学意义（P<0.05），各组毒性评级均为一级。倒置显微镜下可见加入低浓度金纳米花粒子的人成纤维细胞形态正常，生长良好。结论：此种具有近红外吸收功能的金纳米花粒子体外细胞毒性具有剂量依赖性，低浓度金纳米花粒子具有良好的生物相容性。

Cytotoxicity of gold nano-flower particles to human fibroblasts in vitro

Objective To investigate the cytotoxicity of gold nano-flower particle which has the absorption ability in near-infrared(NIR) region to human fibroblasts(HF) in vitro,and to provide basis for its application in biomedical field.Methods HF were cultured with medium contained different doses of gold nano-flower particles(1.875,3.750,7.500,15.000,30.000 mg·L-1) for 24 h,the HF cultured with medium free of nanoparticles as control group,the cytotoxicity effect was tested by MTT method,the morphology of HF was observed under inverted microscope.Results The relative growth rates(RGR)were 92.245%,88.980%,86.939%,75.782% and 71.756% respectively in different doses of gold nano-flower particle groups,the RGR in 30.000 mg·L-1 group had significant difference compared with control group(P<0.05),while all groups were the first cytoxicity grade.The HF had normal morphology and well growth under inverted microscope observation in low dose gold nano-flower particle group.Conclusion The cytotoxicity of the gold nano-flower particles has does-effect response,and low dose of gold nano-flower particles have well biocompatibility with HF.