乳胞素对胶质瘤C6细胞体内增殖的抑制作用

目的：在体外实验蛋白酶体抑制剂乳胞素(LAC)可以抑制神经胶质瘤C6细胞增殖的基础上，进一步在裸鼠体内观察乳胞素对C6细胞增殖率的影响，探讨乳胞素抑制胶质瘤细胞增殖率的可能机制。方法：体外培养C6细胞，选择处于对数生长期的细胞接种于裸鼠背部皮下，连续7d腹腔注射给予乳胞素，实验分为模型组(Model)、乳胞素0.5 μ...

Inhibitory effect of lactacystin on proliferation of glioma C6 cells in vivo

Objective To observe the effect of lactacystin(LAC) on the proliferation rate of C6 cells in vivo on the basis of inhibitory effect of LAC on C6 cells in vitro and to discuss the possible mechanism of LAC inhibiting the proliferation rate of C6 cells.Methods The C6 cells were cultivated in vitro,and the cells in the logarithmic growth phase were selected and were inoculated in nude mice subcutaneously.All mice were given LAC for 7 d.They were divided into model group,LAC 0.5 μg / 20 g group,LAC 1 μg / 20 g group,and the LAC 5 μg / 20 g group.There were three nude mice in each group.The general condition of mice(food,bathroom,coat color,activities,etc.) was observed;the tumor volumes at different time points were measured and calculated.The Bax,bcl-2 and caspase-3 mRNA expression levels were detected by RT-PCR method.Results Compared with model group,the tumor volumes in LAC 0.5 μg / 20 g group,LAC 1 μg / 20 g Group,and LAC 5 μg / 20 g group were significantly reduced(P<0.05);the Bax/bcl-2 and caspase-3 mRNA expression levels were increased significantly(P<0.05).There was not statistically significant between different doses of LAC in tumor volume,level of Bax/bcl-2 mRNA and level of caspase-3 mRNA,respectively.Conclusion LAC in animals can significantly inhibit the proliferation rate of C6 cells and its mechanism may be that it increases apoptosis of mitochondrial pathway in C6 cells to inhibit the proliferation.