发酵轮叶党参提取物清除DPPH自由基的作用

目的:探讨发酵和未发酵轮叶党参提取物清除1,1-二苯基苦基苯肼(DPPH)自由基(DPPH·)的能力,阐明酵母菌发酵对轮叶党参清除自由基能力的影响.方法:实验用活性酵母发酵轮叶党参7d后用沸水提取,并用80％乙醇除去蛋白质和多糖,减压浓缩,采用清除DPPH·能力的方法比较发酵、未发酵轮叶党参提取物及抗坏血酸对DPPH·...

DPPH free radical scavenging ability of fermented Codonopsis lanceolata extract

Objective To study comparatively the free radical scavenging effects of fermented and non-fermented Codonopsis lanceolata(CL) extract,and elucidate the effect of active yeast on free radical scavenging activity of CL.Methods CL was fermented with active yeast for 7 d,then extracted with boiling distilled water,protein and polysaccharide were removed with 80% ethanol,vaccum concentrated.Free radical scavenging abilities of fermented,non-fermented CL extract and Vc were studied by 1,1-diphenyl-2-picryl-hydrazyl(DPPH) scavenging assay,simultaneously the oleanolic acid content in the extracts was determined.Results The EC50 values of fermented and non-fermented CL extract were 0.12 g·L-1 and 0.40 g·L-1,respectively.The DPPH· free radical scavenging ability of fermented CL extract was significantly higher than that of non-fermented CL extract(P<0.01).After fermentation,the oleanolic acid content in CL was increased 1.41 times.Conclusion DPPH· free radical scavenging effects of CL are significantly increased after fermentation with active yeast,and its chemical composition changes after fermentation.