黄芪对支气管哮喘大鼠支气管肺泡灌洗液及血清中NO、IL-5和炎症细胞的影响

目的:探讨黄芪对支气管哮喘模型大鼠血清及支气管肺泡灌洗液(BALF)中一氧化氮(NO)、白细胞介素5 (IL-5)和炎症细胞的影响,阐明黄芪对哮喘的治疗效果.方法:60只Wistar大鼠随机分为正常对照组、模型组、激素干预组及黄芪干预低、中、高剂量组,每组10只,除正常对照组外以卵白蛋白(OVA)致敏并吸入激发法制备大...

Effect of mongolian milkvetch root on NO, IL-5, inflammatory cells in BALF and serum of bronchial asthmatic rats

Objective To observe the effect of mongolian milkvetch root on nitric oxide(NO),interleukin 5(IL-5),inflammatory cells in bronchoalveolar lavage fluid(BALF) and serum of bronchial asthmatic rats and clarify the efficacy of mongolian milkvetch root in treatment of asthma.Methods 60 Wistar rats were randomly divided into normal group,model group,budesonide suspension for inhalation intervention group and low,middle,and high dose mongolian milkvetch root intervention groups,10 rats in each group.The rat bronchial asthma models were established by egg protein(OVA) sensibilization and long-term inhalation provocation.The rats in mongolian milkvetch root intervention groups were lavaged with mongolian milkvetch root 2 mL(0.3 g·100g-1),and the rats in budesonide suspension for inhalation intervention group were inhalated with budesonide suspension 2 mL at 21 d after expreriment;the rats in normal group and model group were lavaged with normal saline orally,once each day for 14 d.The behavior of rats was observed and the changes of NO,IL-5,inflammatory cells in BALF and serum of bronchial asthmatic rats in each group were detected.Results The model of asthma was set up successfully by observing the behavior of rats,then the behavior of the rats became better after treatment,but did not reach the normal state.The NO and IL-5 levels in BALF and serum in mongolian milkvetch root intervention groups were significantly reduced compared with model group(P<0.01),and the percentages of the total inflammatory cells and eosinophils in BALF were significantly reduced(P<0.01).Conclusion Mongolian milkvetch root could inhibit the increasing of IL-5 and NO in BALF in asthmatic models,and could significantly reduce activation and infiltration of airway inflammatory cells and eosinophils,thus reduce airway inflammation and asthma symptoms or avoid the onset of asthma to achieve the purpose of prevention and treatment of asthma.