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| 羟氯喹治疗口腔扁平苔藓前后的唾液双向电泳研究 | |
| 引用本文： | 蒋红柳,王文梅,段宁,王翔. 羟氯喹治疗口腔扁平苔藓前后的唾液双向电泳研究[J]. 临床口腔医学杂志, 2010, 26(3): 143-145 |
| 作者姓名： | 蒋红柳 王文梅 段宁 王翔 |
| 作者单位： | 南京大学医学院附属口腔医院,江苏,南京,210008 |
| 基金项目： | 南京市人才项目资助(200603005) |
| 摘 要： | 目的：比较口腔扁平苔藓（OLP）经羟氯喹治疗后,唾液中差异表达的蛋白质,探讨羟氯喹治疗OLP的可能作用机制。方法：采用固相pH梯度（immobilized pH gradient,IPG）双向凝胶电泳（two-dimensional gel electrophoresis,2-DE）分离OLP经羟氯喹治疗三个月后唾液中的差异蛋白质,银染显色,Imagemaster图像分析软件分析差异蛋白质点。结果：①羟氯喹治疗OLP前和治疗后的唾液2-DE图谱的平均蛋白质点数分别为1894±183个和1920±341个,凝胶图谱分辨率、重复性较好;②通过Imagemaster图像分析比较治疗前后的唾液2-DE图谱,差异表达的蛋白质点数为21个,其中6个点在治疗3个月后高表达,15个点在治疗后低表达。结论：本研究建立了分辨率高且重复性较好的OLP治疗前后的唾液双向凝胶电泳图谱,发现存在差异表达的蛋白质,为进一步研究羟氯喹治疗OLP的作用机制奠定了基础。 |
| 关 键 词： | 口腔扁平苔藓 唾液 羟氯喹 双向凝胶电泳 蛋白质组 |
saliva two-dimensional gel electrophoresis analysis about hydroxychloroquine action on oral lichen planus. |
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| JIANG Hong-liu,WANG Wen-mei,DUAN Ning,WANG Xiang. saliva two-dimensional gel electrophoresis analysis about hydroxychloroquine action on oral lichen planus.[J]. Journal of Clinical Stomatology, 2010, 26(3): 143-145 | |
| Authors: | JIANG Hong-liu WANG Wen-mei DUAN Ning WANG Xiang |
| Affiliation: | JIANG Hong-liu,WANG Wen-mei,DUAN Ning,WANG Xiang.The Stomatological Hospital Affiliated to Medical College of Nanjing University,Jiangsu Nanjing 210008,China. |
| Abstract: | Objective:To compare the difference of the serum protein about the patient of oral lichen planus who received hydroxychloroquine therapy and to explore its possible mechanism.Method:The total serum proteins of oral lichen planus who received hydroxychloroquine therapy were separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis(2-DE).After AgNO3 staining,the differential expression proteins were ana1yzed by using ImageMaster image ana1ysis software.Result:(1)The well-resolved... |
| Keywords: | oral lichen planus saliva hydroxychloroquine two-dimensional gel electrophoresis proteome |
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