Use of pyrosequencing to explore the benthic bacterial community structure in a river impacted by wastewater treatment plant discharges |
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Affiliation: | 1. UTA-RAM-OneHealth Group, Centro de Investigaciones Agropecuarias, Facultad de Ciencias Agropecuarias, Universidad Técnica de Ambato, Tungurahua, Ecuador;2. Catalan Institute for Water Research (ICRA), University of Girona, Girona, Spain;1. College of Resources Environment and Tourism, Capital Normal University, Beijing 100048, China;2. Beijing Municipal Key Laboratory of Resources Environment and GIS, Beijing 100048, China;3. Urban Environmental Processes and Digital Modeling Laboratory, Beijing 100048, China;4. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China |
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Abstract: | In this study, we determined the diversity and composition of benthic bacterial communities collected in river sediments upstream and downstream from a wastewater treatment plant (WWTP). Pyrosequencing of bacterial 16S rRNA genes revealed notable differences between the communities from upstream and downstream sites. In particular, a higher relative abundance of Acidobacteria, Chloroflexi, Deltaproteobacteria and Firmicutes and a lower proportion of Gammaproteobacteria and Verrucomicrobia sequences were detected at the downstream site compared to the upstream site. These findings represent a first approximation of the impact of WWTP discharges on environmental microbial communities. |
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Keywords: | Wastewater treatment plant Microbial diversity Sediment Aquatic ecosystem |
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