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| in vitro differentiation of adipose-derived stem cells into hair cell-like cells in guinea pigs | |
| 引用本文： | WANG Ying1,DONG Min-min1,YANG Hai-bo2,GAO Song-tao3 1 Department of Otolaryngology,the First Affiliated Hospital of Zhengzhou University,China 2 Department of Cardiology,The First Affiliated Hospital of Zhengzhou University,China 3 Department of orthopaedics,Xiangya Hospital of Central South University,China. in vitro differentiation of adipose-derived stem cells into hair cell-like cells in guinea pigs[J]. 中华耳科学杂志(英文版), 2007, 2(2): 97-101. DOI: 10.1016/S1672-2930(07)50020-6 |
| 作者姓名： | WANG Ying1 DONG Min-min1 YANG Hai-bo2 GAO Song-tao3 1 Department of Otolaryngology the First Affiliated Hospital of Zhengzhou University China 2 Department of Cardiology The First Affiliated Hospital of Zhengzhou University China 3 Department of orthopaedics Xiangya Hospital of Central South University China |
| 作者单位： | WANG Ying1，DONG Min-min1，YANG Hai-bo2，GAO Song-tao3 1 Department of Otolaryngology，the First Affiliated Hospital of Zhengzhou University，China 2 Department of Cardiology，The First Affiliated Hospital of Zhengzhou University，China 3 Department of orthopaedics，Xiangya Hospital of Central South University，China |
| 摘 要： | Objective Adipose-derived stem cells(ADSCs) are suggested to possess a highly plastic ability to differentiate into several specific cell types in addition to adipocyte lineages, including germ layer tissue-specific cell lineages such as chondrocyte, myocyte, neuronal, and osteoblast lineages. The aim of this study is to establish an in vitro culture technique for ADSCs in an adult guinea pig model that facilitate their differentiation into hair cell-like cells. Materials and Methods Cells from inguinal fat pads in adult guinea pigs were cultured with β-mercaptoethanol, RA, Forskorin, Heregulin, bFGF, BDNF and EGF. Cellular differentiation was examined using immunocytochemistry techniques. Results The ADSCs demonstrated hair cell immunophenotypes with expression of epitopes of the hair cell marker protein myosin Ⅶa. Conclusion ADSCs from adult guinea pig adipose tissue can differentiate into hair cell-like cells when cultured in vitro. ADSCs may serve as seed cells for tissue engineering. |
| 收稿时间： | 2007-11-11 |
In vitro Differentiation of Adipose-Derived Stem Cells into Hair Cell-Like Cells in Guinea Pigs |
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| WANG Ying,DONG Min-min,YANG Hai-bo,GAO Song-tao. In vitro Differentiation of Adipose-Derived Stem Cells into Hair Cell-Like Cells in Guinea Pigs[J]. Journal of Otology, 2007, 2(2): 97-101. DOI: 10.1016/S1672-2930(07)50020-6 | |
| Authors: | WANG Ying DONG Min-min YANG Hai-bo GAO Song-tao |
| Affiliation: | 1. Department of Otolaryngology, the First Affiliated Hospital of Zhengzhou University, China;2. Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, China;3. Department of orthopaedics, Xiangya Hospital of Central South University, China |
| Abstract: | Objective Adipose-derived stem cells (ADSCs) are suggested to possess a highly plastic ability to differentiate into several specific cell types in addition to adipocyte lineages, including germ layer tissue-specific cell lineages such as chondrocyte, myocyte, neuronal, and osteoblast lineages. The aim of this study is to establish an in vitro culture technique for ADSCs in an adult guinea pig model that facilitate their differentiation into hair cell-like cells. Materials sad Methods Cells from inguinal fat pads in adult guinea pigs were cultured with β -mercaptoethanol, RA, Forskorin, Heregulin, bFGF, BDNF and EGF. Cellular differentiation was examined using immunocytochemistry techniques. Results The ADSCs demonstrated hair cell immunophenotypes with expression of epitopes of the hair cell marker protein myosin Ⅶa. Conclusion ADSCs from adult guinea pig adipose tissue can differentiate into hair cell-like cells when cultured in vitro. ADSCs may serve as seed cells for tissue engineering. |
| Keywords: | adipose-derived stem cell hair cell differentiate guinea pig |
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