汞化合物对人体外淋巴细胞的DNA损伤的比较研究

对人体外淋巴细胞用不同浓度的甲基氯化汞（ＭＭＣ）和氯化汞（ＭＣ）处理１ｈ，观察ＤＮＡ损伤的程度，采用细胞琼脂糖凝胶电泳技术（ＳＣＧＥ）进行比较研究。这两种汞化物均不同程度的显示了剂量反应关系。ＤＮＡ损伤的程度从１０－５ｍｏｌ／Ｌ起与空白对照相比，呈现有意义的增加，细胞ＤＮＡ断裂频率的增加与毒性呈现为线性增长的关系。剂量愈高，则ＤＮＡ的彗尾链愈长，两种化合物在同样的实验条件下显示了相似的分裂剂的潜能，氯化汞表现出的遗传毒性更明显

Comparison Study of DNA Breakage Capacity of Mercury Compounds in vitro in Human Lymphocytes

Human lymphocytes were treated in vitro for 1h with methylmercury chloride(MMC) and mercury chloride (MC).The extend of induced DNA fragmentation was measured with a single cell microgel electrophoesis assay(SCGE).Both compounds showed a dose dependent cytotoxic effects.There are a significant increase of DNA damage from 10 -5 mol/L treatments compare to negative control(P<0 01).The frequency of highly damaged cells increased almost linearly with toxicity.The higher concentration of the test chemicals,the greater was the spreading of tail lengths.Both compounds have shown a similar clastogenic potential in the same test condition.Mercury chloride shown more strong genotoxic effect.