褐藻素通过PI3K/AKT信号通路抑制食管鳞癌细胞迁移和侵袭

目的 研究褐藻素对人食管鳞癌EC109和EC9706细胞迁移和侵袭的影响,并初步探究其作用机制.方法 采用四甲基偶氮唑蓝(MTT)法检测不同浓度的褐藻素对食管鳞癌细胞活性的影响;采用划痕实验、Transwell迁移和侵袭实验分别考察褐藻素对食管鳞癌细胞迁移和侵袭能力的影响;使用Western blotting实验检测褐...

Fucoxanthin Suppresses the Migration and Invasion Through PI3K/AKT Signaling in Esophageal Squamous Cell Carcinoma Cells

OBJECTIVE To explore the inhibitory effects of fucoxanthin on the migration and invasion of ESCC cells (EC109 and EC9706), and elucidate the underlying molecular mechanisms. METHODS MTT assay was used to detect the cell viability of ESCC cells. Wound healing, Transwell migration and invasion assays were performed to assess the effect of fucoxanthin on migration and invasion in ESCC cells. Western blotting was employed to detect the protein levels of PI3K, p-PI3K (Tyr607), AKT, p-AKT (Ser473), MMP-2, MMP-9, N-Cadherin and E-Cadherin. RESULTS Fucoxanthin inhibited the viability of EC109 and EC9706 cells,with IC₅₀ values of (51.49±4.15) and (52.44±3.38) μmol·L^-1, respectively. Interestingly, fucoxanthin also dose-dependently suppressed the migration and invasion of EC109 and EC9706 cells, and downregulated the protein levels of MMP-2, MMP-9 and N-Cadherin and enhanced the expression of E-Cadherin. Further investigations showed that the protein levels of p-PI3K (Tyr607) and p-AKT (Ser473), but not the total protein of PI3K and AKT, were substantially suppressed upon fucoxanthin treatment. CONCLUSION Fucoxanthin can drastically diminish the migration and invasion of ESCC cells, and the underlying molecular mechanism might be blocking PI3K/AKT signaling pathway.