宫颈上皮内瘤变与人类白细胞抗原及人乳头瘤病毒感染相关性的研究

目的:探讨宫颈上皮内瘤变(CIN)与人乳头瘤病毒(HPV)感染及人类白细胞抗原(HLA)的相关性。方法:选取宫颈活检组织病理学诊断为CINⅠ42例、CINⅡ40例、CINⅢ29例、宫颈湿疣40例、慢性宫颈炎90例,从液基薄片检测剩余的保存液中提取宫颈脱落细胞学的基因组DNA,采用PCR方法及HLA试剂盒进行HPV16/18及HLA分型检测。结果:HPV16/18检出率CINⅠ组为21.4%,CINⅡ组为47.5%,CINⅢ组为55.2%,湿疣组为32.5%,宫颈炎组为1.1%。HLA分型表明,DR15(2)、DQ6(1)、DQ4在CIN组中阳性率分别为37.0%、44.0%、12.0%,明显高于宫颈炎组的17.5%、18.8%、3.8%(P<0.05),并且DR4阳性率随着CIN级别增高而增高(CINⅠ5.6%,CINⅡ13.9%、CINⅢ28.6%)。结论:HPV16/18感染和HLA分型与CIN及其分级密切相关,提示HLA在宫颈癌的发生、发展中可能起一定的作用。

Study on the correlations of cervical intraepithelial neoplasia with human leucocyte antigens and human papillomavirus infection

Objective:To explore the correlations of cervical intraepithelial neoplasia(CIN) with human leukocyte antigens(HLA) and human papillomavirus(HPV)infection.Methods:The DNA of cast-off cells from cervix uteri was extracted in 42CINⅠ、40CINⅡ、29CINⅢ,40HPV infection and 90 cervicitis that had diagnosed by pathologist.MY09/MY11 and HPV16/18,the consensus and the type specific primers of HPV were employed in amplification of polymerase chain reaction(PCR).The HLA detection kit was used to determinate HLA type.Resulsts:The positive rate of HPV16/18DNA was more higher in CINⅠ(21.4%)、CINⅡ(47.5%)and CINⅢ(55.2%)than that in cervicitis(1.1%).By using HLA kit to type HLA,the positive rates of DR15(2),DQ6(1) and DQ4 were 37.0%,44.0% and 12.0% in CIN respectively,which were higher than those in cervicitis(17.5%,18.8% and 3.8% respectively).The positive rate of DR4 HLA increased with elevation of CIN grade.Conclusions:There are definite correlations between CIN and the infection of HPV16/18.The types of HLA are certainly associated with CIN,suggesting that the hereditary susceptibility is also involved in the occurrence of CIN.