异丙酚及依托咪酯对兔外周血中PLA2活性影响的研究

目的 探讨麻醉药物对正常动物外周血中PLA2的活性及其他炎症因子的影响. 方法 选取24只新西兰大白兔分为三组：对照组、异丙酚组和依托咪酯组,每组8只.分别给予生理盐水对照及临床剂量的异丙酚和依托咪酯,在给药前（T0）、麻药诱导后10 min（T1）、维持剂量后60 min（T2）、120 min（T3）及停止用药后60 min（T4）、120 min（T5）多个不同时间段收集兔外周血,采用荧光底物激发方法或ELISA法分别测量不同组、不同时间段的外周血PLA2活性及炎症因子IL-6、TNF-α、CRP的浓度,观察不同组内PLA2活性及炎症因子的浓度随时间变化的趋势,分析组内、组间的差异性及各炎症因子变化的相关性. 结果 依托咪酯能促进正常动物外周血中PLA2活性炎症因子IL-6、TNF-α、CRP浓度的升高,T3与T0时比较,差异有统计学意义（P＜0.05）;而异丙酚在T3时显著抑制了血清中PLA2的活性及CRP的浓度,但IL-6及TNF-α的浓度未见明显变化.炎症因子之间的相关性分析显示,PLA2的活性变化与CRP的浓度具有一致性（r=0.686,P=0.014）,而与IL-6、TNF-α无相关性. 结论 依托咪酯促进正常机体外周血中炎症因子的活化及释放,而异丙酚表现出一定的抑制作用.

Effect of propofol and etomidate on activation of PLA2 in peripheral blood of rabbits

Objective To investigate the effect of propofol and etomidate on the activation of phosphohpase A2 （PLA2） and the expression of interleukin-6 （IL-6）,tumor necrosis factor-α （TNF-α）,and C-reaction protein （CRP） in rabbit peripheral blood.Methods Twenty-four New Zealand white rabbits were randomly divided into 3 groups with 8 animals in each group：control group,propofol group,and etomidate group.Normal saline,clinical doses of propofol and etomidate were intravenously infused in three groups,respectively.Peripheral blood of each rabbit was collected before anaesthetic injection （T0）,10 min （T1） after anesthesia induction,60 min（T2） and 120 min（T3） after anesthesia,60 min（T4） and 120 min（T5） after drug deprivation.PLA2 activities were analyzed using the fluorescent substrate DBPC,a fluorogenic phosphatidylcholine substrate.ELISA was performed to detect the expression of other inflammatory cytokines.The difference of PLA2 activity and expression of inflammatory cytokines at different time points were statistically analyzed.Results Etomidate significantly induced PLA2 activities and increased the expression of IL-6,TNF-α,and CRP at T3 （P ＜0.05）,while propofol inhibited serum PLA2 activities and expression of CRP at T3 but had no effect on IL-6 and TNF-α in peripheral blood of the rabbits.PLA2 activity was positively correlated with expression of CRP （r =0.686,P =0.014）,but not to IL-6 and TNF-α.However,the expression of IL-6 and TNF-α was not relevant.Conclusion Etomidate can induce the activation and expression of multiple inflammatory cytokines in peripheral blood,however propofol has the opposite effects.