布鲁杆菌病患者体内T细胞免疫功能研究

目的探讨布鲁杆菌病人体内T淋巴细胞及相关细胞因子的变化。方法用流式细胞术检测外周血单个核细胞中CD4^＋T细胞、CD8^＋T细胞和CD4／CD8比例；用ELISA法检测血清中IFN-γ、IL-2、IL-4和IL-5。结果试验组外周血CD4^＋T细胞和CD4／CD8比例明显高于对照组（P〈0．05），而CD8^＋T细胞与对照组相比无显著差异（P〉0．05）；试验组血清中IFN-γ和IL-2水平明显高于对照组（P〈0．05），而IL-4和IL-5水平与对照组相比无显著差异（P〉0．05）。结论布鲁杆菌感染后T细胞免疫主要表现为Th1功能增强，这可能对机体清除病原菌有重要意义。

Research on T cells immune function in brucellosis patients

Objective To investigate changes of T cell subsets and related cytokines in brucellosis patients. Methods Proportions of CIM^＋ T cells, CD8^＋ T cells and CIM/CD8 ratio in peripheral blood mononuclear ceils （ PBMC ） were detected by flow cy-tometry ; serum IFN-γ, IL-2, IL-4 and IL-5 were detected by ELISA. Results Proportion of CI4^＋ T cells and CIM/CD8 ratio in experimental group were significantly higher than that in control group （ P 〈0. 05 ）. Proportion of CD8 ^＋ T cells was similar intwo groups （ P 〉0. 05 ）. Levels of IFN-γ and IL-2 in experimental group were significantly higher than that in control group （ P 〈0. 05 ）. Levels of IL-4 and IL-5 were similar in two groups （ P 〉0. 05 ）. Conclusions Enhancement of Thl cells function was the main change of T cell immune after brucellosis infection ,which may play an important role in removing pathogen out of body.