MMP-1基因多态性与舌癌发生及预后的相关研究

目的探讨基质金属蛋白酶1（MMP-1）基因-16071G／2G多态性与舌癌发生及预后之间的关系。方法以中国北方地区汉族人群为研究对象进行病例对照研究，选择98例舌癌患者为病例组，100例性别、年龄相匹配的健康者为对照组。应用聚合酶链反应-限制性片段长度多态性PCR-RFLP分析方法对MMP-1-16071G／2G单核苷酸多态性进行基因分型。结果病例组和对照组中MMP-1-16071G／2G基因型分布有显著差异（χ2=6．748，df=2，P=0．034），等位基因分布无显著差异（χ2=1．538，df=1，P=0．215）。各基因型发生舌癌风险无显著差异，各等位基因间发生舌癌风险也无显著差异。但携带基因型1G／2G和2G／2G的患者较基因型1G／1G舌癌复发风险显著升高（OR=4．762，95％CI=1．106-20．501，P=0．036：OR=6．667，95％CI=1．619-27．456，P=0．009）；携带等位基因[2G]较等位基因[1G]舌癌复发风险增加2．397倍（OR=2．397，95％CI=1．290-4．457，P=0．006）。等位基因[2G]与MMP-1的表达呈正相关。结论MMP-1-16071G／2G多态性可能是舌癌复发的危险因素。

Association between MMP-1 -1607 1G/2G single nucleotide polymorphism and the incidence and prognosis of tongue cancer

Objective To investigate the relationship between the polymorphism of MMP-1 - 1607 1G/2G and the incidence and prog- nosis of tongue cancer. Methods In a case-control study for the Chinese population in northern China,98 patients with tongue cancer and 100 age- and sex-matched healthy subjects were enrolled. The single nucleotide polymorphism （MMP-1 - 1607 1G/2G） was geno- typed by polymerase chain reaction-restriction fragment length polymorphism （PCR -RLFP） technique. Results There was a statis- tically significant difference in the genotypic distribution of MMP-1 - 1607 1G/2G between the cases and the controls, but not in the al- lele frequency of MMP-1 - 1607 1G/2G. No significant relationship between MMP-1 - 1607 IG/2G and the occurence of tongue cancer was detected. However,the risk of recurrence significantly increased in the patients with 1G/2G and 2G/2G genotype（ OR = 4. 762, 95 % CI = 1.106 -20.501, P = 0. 036 ; OR = 6. 667,95 % C I = 1. 619 - 27. 456, P = 0. 009） compared with those with 1 G/I G genotype. Compared to the patients carrying allele 1G,the risk of recurrence of those with allele 2G increased 2. 397 times （95% CI = 1. 290 - 4.457,P = 0. 006）. Allele 2G was positively correlated with the expression of MMP-1. Conclusion MMP-1 - 1607 1G/2G polymor- phism may be a risk factor for the recurrence of tongue cancer.