维甲酸诱导BALB／c近交系小鼠腭裂动物模型的实验研究

目的分析维甲酸（RA）在胚胎发育期间对胎鼠的致畸作用，建立维甲酸诱导腭裂动物模型的最佳剂量。方法将特定妊娠期BALB／c近交系小鼠分为对照组和实验组。实验组小鼠一部分在妊娠8d，10d，12d管饲RASOmg／kg，一部分在妊娠10d给予40mg／kg、50mg／kg和60mg／kg三种不同剂量RA；对照组小鼠在妊娠8d，10d，12d管饲等剂量玉米油。观察胎鼠腭裂比率，确定诱导腭裂形成的最佳作用条件。结果RA在不同的发育阶段均可诱导腭裂，妊娠第10天50mg／kgRA诱导腭裂的比率为93．75％（P〈0．05），诱导腭裂形成的比率最高。结论RA诱导BALB／c近交系小鼠腭裂是一种较为理想的腭裂动物模型，其最佳剂量为妊娠第10天给予50mg／kgRA。

Study on retinoic acid-induced cleft palate model in BALB/c mice

Objective To explore the toxicity of retinoic acid（RA） to fetus and the optimal dose of RA for the cleft palate model in BALB/c mice. Methods Pregenant mice at specific period were divided into two groups：control group and experiment group. Part of the pregnant mice were respectively treated with 50 mg/kg RA at GD8 d,GD10 d and GD12 d in experiment groups,and part of the pregnant mice were respectively treated with 40 mg/kg,50 mg/kg and 60 mg/kg RA at GD10 d 40 mg/kg,50 mg/kg and 60 mg,/kg RA. The pregnant mice received the same volume of corn oil in control group. The incidence of embryo cleft palate was observed at the different doses and different feeding time to determine the optimal conditions of inducing cleft palate model. Results RA induced cleft palate at all the different developmental stages. The rate of RA-induced cleft palate was 93.75% at the exposed dose of 50 mg/kg at GD 10 d. Conclusion It is an ideal model for cleft palate induced by 50 mg/kg RA at GD10 d.