甘草酸对实验性梗阻性肾病大鼠的保护作用

目的　研究甘草酸对实验性梗阻性肾病大鼠是否具有保护作用。方法　结扎大鼠单侧输尿管建立梗阻性肾病模型 ,分别于术后 12h ,3、14、2 8和 5 6d处死实验动物 ,切取肾皮质部位组织 ,光、电镜观察肾间质病理形态学变化并进行有核细胞计数 ;免疫组织化学染色检测肾间质Ⅰ、Ⅲ型胶原蛋白表达 ,并用EIG综合图像分析系统对其表达进行半定量分析。结果　模型显示肾间质呈进行性纤维化。术后 12h ,甘草酸治疗组和预防治疗组与假手术组比较 ,肾间质有核细胞计数显著增多 (P <0 .0 5 ) ,与生理盐水组比较显著减少 (P <0 .0 1) ,但在甘草酸治疗组和预防治疗组差异无显著性 (P >0 .0 5 )。半定量分析发现 ,肾间质Ⅰ型胶原蛋白的表达 ,在生理盐水组于术后 12h和假手术组比较差异有显著性 (P <0 .0 5 ) ;术后 3d ,甘草酸治疗组和预防治疗组肾间质Ⅰ型胶原蛋白的表达也高于假手术组 (P <0 .0 1) ,但显著低于生理盐水组 (P <0 .0 1) ,而甘草酸治疗组和预防治疗组差异无显著性 (P >0 .0 5 ) ,Ⅲ型胶原蛋白的表达与Ⅰ型胶原蛋白的表达基本一致。结论　甘草酸对大鼠梗阻性肾病具有一定的治疗作用 ,但无预防作用。

Protective role of glycyrrhizin on experimental obstructive nephropat hy in rats

Objective　To investigate the protective role of glycyrrhizin on experimental obstructive nephropathy in rats. Methods 　The model of obstructive nephropathy was induced by unilateral uretera l ligation in rats and the animals were sacrificed at 12 h and 3,14,28,56 d resp ectively after operation. Specimens were taken from the cortex of kidney. In re nal stroma, routine morphological examinations and counts of karyocyte were made , the expression of collagen type Ⅰand type Ⅲ were also detected by immunohist ochemical staining,then, semi-quantified by EIG image analysis system. Results　 A progressive fibrosis was observed in renal stroma of the mod el. 12 h after operation, counts of karyocyte increased markedly i n renal stroma in groups of treatment and pretreatment with glycyrrhizin compar ed with that in sham-operation group（P<0.05）,and decreased distinctly compared with that in saline treated group（P<0.01）, but no significant di fference was found between treatment group and pretreatment group（P>0.05） ;3 d after operation, in group treated or pretreated with glycyrrhizin. The e xpression of collagen type Ⅰ increased markedly compared with that in sham-o peration group（P<0.01） in renal stroma, but decreased notably compare d with that in saline treated group（P<0.01）,while, there was not any diffe rence between treatment group and pretreatment group（P>0.05）. The expressi on of collagen type Ⅲ in renal stroma was almost the same as that of coll agen type Ⅰ. Conclusion　Glycyrrhizin has some therapeutical bu t no preventive effect to experimental obstructive nephropathy in rats.