bFGF和顺铂、米托蒽醌及阿糖胞苷对兔晶状体上皮细胞的相互作用

目的 观察碱性成纤维细胞生长因子（bFGF)和顺铂（PDD）、米托蒽醌（Mit)及阿糖胞苷（Ara-C)对兔晶状体上皮细胞（RLECs)的相互作用。方法 分离兔晶状体囊膜行组织细胞培养，传代的2-3代RLECs在96孔板培养24h，然后分别用不同浓度的bFGF,PDD,Mit和Ara-c;以及bFGF合用PDD，Mit及Ara-C作用24h及72h，用（MTT）比色法测定对RLECs的影响。结果 bFGF可明显促进晶状体上皮细胞的增殖，而PDD和Mit明显抑制晶状体上皮细胞的增殖，在bFGF存在的情况下此两种药物仍能抑制晶状体上皮细胞的增殖，在本实验条件下Ara-C单用对兔晶状体上皮细胞无明显影响。而在bFGF存在时则表现出抑制作用。结论 bFGF是促进兔晶状体上皮细胞增殖的重要因子。并不能阻止抑制兔晶状体上皮细胞增殖药物对其的抑制作用。而对z细胞周期特异性药物的作用有增强效果。

Mutual effect of bFGF and Cisplatin,Mitoxantrone,Cytosine Arabinoside on rabbit lens epithelial cells

Objective To observe the mutual effect of bFGF and Cisplatin, Mitoxantrone, Cytosine Arabinoside on rabbit lens epithelial cells. Methods Lens epithelium was isolated and cultured,and RLECs at passages 2 or 3 were placed in 96-well tissue culture plates and incubated for 24 hours,they were then exposed to different concentrations individually or as combination of bFGF, Cisplatin, Mitoxantrone and Cytosine Arabinoside for 24 and 72 hours and the effect of exposure of RLECs to the above was measured by a rapid colorimetric assay (MTT assay). Results After the treatment with bFGF, RLECs showed marked proliferation, and with PDD and Mit showed marked inhibition while,Ara-c showed no observable effect on RLECs Under this experimental condi- tions. Meanwhile the bFGF excluded,PDD, Mit and Ara-c also showed marked inhibition. Couclusions bFGF is an important factor that can promote proliferation of RLECs. bFGF can not prevent that drugs inhibiting the proliferation of RLECs,and can en- hance special cycle drugs inhibition.