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| 前列腺癌与良性前列腺增生中NFKBIA及SREBF2的表达 | |
| 引用本文： | 江先汉,毕学成,何慧婵,韩兆冬,叶永康,曾国华,钟惟德. 前列腺癌与良性前列腺增生中NFKBIA及SREBF2的表达[J]. 中华实验外科杂志, 2010, 27(3). DOI: 10.3760/cma.J.issn.1001-9030.2010.03.021 |
| 作者姓名： | 江先汉 毕学成 何慧婵 韩兆冬 叶永康 曾国华 钟惟德 |
| 作者单位： | 1. 南方医科大学,510515 2. 广州医学院附属广州市第一人民医院泌尿外科 |
| 基金项目： | 广东省自然科学基金，广州市卫生局重点项目，国家高科技863计划重点项目，国家自然科学基金 |
| 摘 要： | 目的 以实时荧光定量聚合酶链反应(PCR)技术研究良性前列腺增生(BPH)与前列腺癌(PCa)组织标本NFKBIA及SREBF2 mRNA相对值,探讨NFKBIA及SREBF2比值在前列腺癌诊断中的特异性意义.方法 通过实时荧光定量PCR检测63例PCa、37例BPH及3例正常前列腺组织NFKBIA及SREBF2的表达,比较其在PCa与BPH组织中定量的差异.结果 与BPH比较,PCa组织NFKBIA及SREBF2 mRNA的定量表达值分别下调0.520和上调2.547,差异有统计学意义(P＜0.05).结论 实时荧光逆转录(RT)-PER定量检测NFKBIA及SREBF2 mRNA为前列腺癌的诊断提供了参考. |
| 关 键 词： | 前列腺癌/肿瘤学 NFKBIA及SREBF2 实时荧光定量PCR 基因表达 |
NFKBIA/SREBF2 mRNA expression in BHP and prostate cancer |
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| JIANG Xian-han,BI Xue-cheng,HE Hui-chan,HAN Zhao-dong,YE Yong-kang,ZENG Guo-hua,ZHONG Wei-de. NFKBIA/SREBF2 mRNA expression in BHP and prostate cancer[J]. Chinese Journal of Experimental Surgery, 2010, 27(3). DOI: 10.3760/cma.J.issn.1001-9030.2010.03.021 | |
| Authors: | JIANG Xian-han BI Xue-cheng HE Hui-chan HAN Zhao-dong YE Yong-kang ZENG Guo-hua ZHONG Wei-de |
| Abstract: | Objective To study the NFKBIA/SREBF2 mRNA expression in BPH and PCa cells,and explore the diagnostic value of NFKBIA/SREBF2 mRNA expression levels in PCa. Methods A sensitive , real-time quantitative PCR assay was developed to compare the expression difference of NFKBIA/SREBF2 mRNA in BPH, PCa and normal prostate tissue cells, by testing 63 samples of PCa, 37 samples of BHP and 3 samples of normal prostate tissue. Results The expression ratio of NFKBIA/SREBF2 in BPH and PCa prostate tissue cells to normal prostate tissue cells was 0. 520 and 2. 547 respectively ( P ＜0. 05 ). Conclusion The results of NFKBIA/SREBF2 mRNA expression detected by QRT-PCR can afford reliable and helpable information for diagnosis of PCa. |
| Keywords: | Prostate cancer/oncology NFKBIA/SREBF2 Real Time RT-PCR Gene expression |
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