MN血型基因分型应用于河源地区临床输血检测

目的 应用基因分型技术鉴定产生抗-M抗体的患者样本MN血型,协助解决MN血型相关的临床输血问题。方法 在血清学鉴定的基础上,采用聚合酶链反应-序列特异性引物(PCR-SSP)基因定型方法对14例产生抗-M抗体样本的MN血型进行基因分型,并与血清学结果比较; 选取M抗原阴性的血液与之进行交叉配血。结果 14例样本基因分型结果与血清学分型结果相符,其中11例为NN型,2例为MN型,1例为MM型。选取M抗原阴性的供血者血液与该14例患者血液配血均相合,患者输血后无输血反应,24 h内血红蛋白上升。结论 MN血型基因分型技术可作为血清学方法的补充,有利于临床输血相关疑难样本的检测。

Genotying of MN Blood Group Applied to Clinical BloodTransfusion in Heyuan Area

Objective To help solve the problem of clinicalblood transfusion related to MN blood group,using genotyping technology to identify MN blood group of the patients' blood sample which exist anti-M antibody.Methods A total of 14 blood samples which exist anti-Mantibody were detected for MN blood group by polymerase chain reaction-sequence specific primers(PCR-SSP)and serologic technique,respectively,and compared the genotyping results with serotyping results.Selected M antigen negative blood for cross matching with 14 cases.Results 14 cases'sgenotyping results and serotyping results were comcidental and in them,NN typeof 11 cases,MN type of 2 cases,MM type of 1 cases.The M antigen negative blood cross matched with 14 cases were compatible.The patients had no blood transfuse reaction after blood transfusion,and the hemoglobin were increase within 24hour.Conclusion Genotyping technology is helpful for detecting complicated samples related to clinical blood transfusion.It can be usefor the compliment of serologic technique.