原发性胆汁性肝硬化患者外周血Th9细胞表达增高及其临床意义

目的 探讨原发性胆汁性肝硬化(primary biliary cirrhosis,PBC)患者外周血Th9细胞所占百分比并初步探讨其临床意义。方法 应用流式细胞术检测PBC患者(n=38)和健康对照者(n=38)外周血Th9细胞所占百分比,利用临床资料对患者进行Mayo评分。采用实时荧光定量PCR法检测患者组和健康对照组外周血单个核细胞(PBMC)中IL-9,PU-1,TGF-β的表达量。并采用ELISA方法检测患者血清中IL-9的表达量。进一步分析Th9细胞百分比与PBC患者生化指标和Mayo评分的相关性。结果 与健康对照组相比,PBC患者Th9细胞百分比增高(t=27.29; P<0.01),且随疾病分期加重逐渐增高。IL-9,PU-1和TGF-β mRNA的表达量显著增高(t=14.69,23.92,10.48; 均P<0.01),且血清中IL-9的表达量也显著增高(t=11.66; P<0.01)。生化指标(ALP,AST,ALT,GGT,TBIL)较健康对照组均显著增高(t=10.94,12.95,10.56,14.92,27.70; 均P<0.01)。Th9细胞百分比与患者Mayo评分、AST,ALT,GGT和TBIL间呈正相关(P<0.05)。结论 Th9细胞可能参与了PBC发病机制,且与疾病的发展相关,这为未来免疫调节治疗靶点的探索提供了新的依据。

Expression of Th9 Cells from Peripheral Blood of PrimaryBiliary Cirrhosis Patients and Its Clinical Significance

Objective To investigate the role of Th9 cellsin primary biliary cirrhosis and its clinical significance.MethodsThe percentages of Th9 cells in patients with PBC(n=38)and healthy controls(n=38)were measured by flow cytometry.Patients clinical datawere collected and Mayo risk scores were calculated.Determined the levels of IL-9,PU-1 and TGF-βmRNA expressions and the protein levels of IL-9 in plasma.Further analyzed the correlation between Th9 cells and clinical parameters.Results Compared to healthy controls,Th9 cells percentagewas higher in PBC patients and increased with disease stages(t=27.29; P<0.01).IL-9,PU-1 and TGF-βmRNA expressions were increased compared with healthy controls(t=14.69,23.92,10.48; all P<0.01)and the protein level of IL-9 was also up-regulated(t=11.66; P<0.01).The clinical parameters(ALP,AST,ALT,GGT and TBIL)were higher than healthy controls(t=10.94,12.95,10.56,14.92,27.70; all P<0.01).Moreover,the percentageof Th9 cells were positively correlated to Mayo risk,ALT,AST,GGT and TBIL(P<0.05).Conclusion Th9 cells may be involved in thepathogenesis of PBC and related to the disease stage,which provides new cluesfor future immunotherapy.