丙酸睾酮对萃取神经修复大鼠坐骨神经缺损的促进作用

目的 观察丙酸睾酮对甘油萃取神经修复大鼠坐骨神经缺损的促进作用.方法 取SD大鼠60只,手术造成左侧坐骨神经1 cm缺损.每组20只.A组:甘油萃取的坐骨神经修复左侧缺损并注射丙酸睾酮(TP)50 g/L,0.1 ml,2次/周;B组仅以甘油萃取的坐骨神经修复缺损;C组以同种异体神经修复.术后16周,比较各组运动神经传导速度恢复率(RRMNCV)和腓肠肌复合动作电位波幅恢复率(RRCMAP);再生有髓神经纤维计数恢复率(RRMFP)及患侧小腿三头肌湿重/健侧小腿三头肌湿重率(ITSWW/UTSWW).结果 术后16周,A、B、c三组RRMNCV分别为(59.06±10.33)%、(45.80±9.05)%、(23.45±4.60)%;RRCMAP分别为(76.64±5.01)%、(64.55±4.20)%、(20.44 ±3.82)%;RRMFP分别为(79.11±2.55)%、(63.67±4.97)%、(29.76±5.68)%;ITSWW/UTSWW分别为(70.37±9.54)%、(56.77±6.08)%、(33.75±7.89)%,三组比较差异有统计学意义(P<0.05).结果 说明A组修复效果最好,B组次之,C最差.结论 丙酸睾酮对甘油萃取神经修复大鼠坐骨神经缺损具有促进作用.

Promotion of testosterone propionate on repair of rat peripheral nerve defects with chemically extracted nerves

Objective To observe the promotion of testosterone propionate on repair of SD rat peripheral nerve defects with chemically extracted nerves. Methods A total of 60 adult formale SD rats were randomly divided into three groups,20 rats in each group. Group A:l. 0 cm sciatic nerve defect of SD rats were bridged by extracted nerves, and at the same time testosterone propionate was injected into the triceps muscle of calf (50 g/L,0.1 ml,BiW). Group B:The nerve defects of SD rats were repaired by extracted nerves,and sodium chloride was injected (0.1 ml,BiW). Group C;the nerve defects were bridged by auto sciatic nerve. The recovery rate of motor nerve conduction velocities (RRMNCV) and the complex muscular action potential (RRCMAP) in each group were measured at the 16th week after operation. The recovery rate of myelinated fiber populations (RRMFP) was tested. The injured side triceps surae wet weight (ITSWW) and uninjured side triceps surae wet weight (UTSWW) in groups A,B and C were compaired. Results At the 16th week, RRMNCV in groups A, B and C were (59. 06 ± 10. 33)% , (45. 80 ± 9.05)% ,and (23.45 ±4. 60)% correspondingly. There were statistical differences among groups A,B and C (P<0.05).RRCMAP in groups A,B and C was (76. 64 ±5. 01)%, (64. 55 ±4. 20)% ,and (20.44 ±3. 82)% correspondingly. There were statistical differences among groups A,B and C (P<0.05). RRMFP in groups A,B and C were (79. 11 ± 2. 55 )% , (63.67 ±4. 97)%,and (29.76 ± 5.68)% correspondingly. There were statistical differences among groups A, B and C (P<0. 05). ITSWW/UTSWW in groups A,B and C was (70.37 ± 9. 54)% , (56. 77 ±6.08)%,and (33.75 ± 7. 89) % correspondingly. There were statistical differences among groups A, B and C (P<0.05 ). The results showed that group A had the best repairing effects,then group B,and group C the worst. Conclusion Chemically extracted nerves by glycerine can be used to repair SD rat sciatic nerve defects,and have a certain effects. Testosterone propionate can promote this effect.