低分子壳聚糖季铵盐对牛血管平滑肌细胞增殖的影响

目的研究低分子壳聚糖季铵盐对血管平滑肌细胞增殖的影响。方法采用体外培养的牛主动脉平滑肌细胞,通过结晶紫染色、MTT法、透射电镜、流式细胞仪等方法,观察低分子壳聚糖季铵盐对血管平滑肌细胞增殖、细胞表型、细胞增殖周期及细胞凋亡的影响。结果低分子壳聚糖季铵盐10、100、200mg·L-1可以呈浓度依赖性地抑制10%小牛血清所致平滑肌细胞增殖,抑制10%小牛血清致平滑肌细胞由收缩型向合成型的转变,低分子壳聚糖季铵盐200mg·L-1具有诱导血管平滑肌细胞凋亡的作用。结论低分子壳聚糖季铵盐可以呈浓度依赖性的抑制血管平滑肌细胞增殖。

Effect of low molecular N-trimethyl chloride chitosan on the proliferation of smooth muscle cells

Aim To investigate the effect of low molecular N-trimethyl chloride chitosan(LMTC) on the growth of bovine vascular smooth muscle cells(BVSMCs) in vitro. Methods The antiproliferation activities of LMTC were evaluated in BVSMCs by means of crystal violet staining and MTT assay. The morphological changes of LMTC in BVSMCs were observed under transmission electron microscope. Cell survival ratio influenced by LMTC was assessed by flow cytometer. [WTHZ]Results After BVSMCs were treated by LMTC for 72 h,the growth of the cells was inhibited in vitro and was dependent on concentration; there were some changes of apoptosis by transmission electron microscope and flow cytometer. Conclusion LMTC can inhibit the proliferation of BVSMCs and induce their apoptosis.