Development and characterization of thirteen polymorphic microsatellite loci in the Cynoglossus semilaevis

Thirteen polymorphic microsatellite loci have been isolated and characterized from Cynoglossus semilaevis for the purpose of protecting its wild resources. They had between three and nine alleles. Observed and expected heterozygosities varied from 0.57 to 0.93, and from 0.56 to 0.82, respectively. All the loci conformed to Hardy–Weinberg equilibrium after Bonferroni correction. No significant linkage disequilibrium was detected among the loci (P > 0.004). They have provided sufficient level of genetic diversity to investigate the fine-scale population structure and species conservation and management in the C. semilaevis.