| 模拟缺血对心室肌细胞钙内流和钾外流的影响 |
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| 引用本文： | 王文伟,龚惠,朱依纯,龚茜玲. 模拟缺血对心室肌细胞钙内流和钾外流的影响[J]. 老年医学与保健, 2003, 9(2): 84-86,110 |
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| 作者姓名： | 王文伟 龚惠 朱依纯 龚茜玲 |
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| 作者单位： | 200072 上海,复旦大学上海医学院生理学教研室,国家医学神经生物学重点实验室;200072 上海,复旦大学上海医学院生理学教研室,国家医学神经生物学重点实验室;200072 上海,复旦大学上海医学院生理学教研室,国家医学神经生物学重点实验室;200072 上海,复旦大学上海医学院生理学教研室,国家医学神经生物学重点实验室 |
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| 基金项目： | 上海市曙光计划项目(项目编号:97SG15) |
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| 摘 要： | 目的 观察模拟缺血对心室肌细胞L—型钙通道和ATP敏感钾通道的影响。方法 实验用胶原配药解法急性分离豚鼠心室肌细胞,利用全细胞膜片钳的方法记录心室肌细胞的L—型钙电流(Ica.L)和ATP敏感钾电流(IKATP)。采用低氧、无糖、高钾、高乳酸和酸中毒综合方式模拟缺血灌流,造成细胞的模拟缺血。结果 模拟缺血时,Ica.L明显受到抑制,IKATP增加,后一效应能被优降糖所阻断。结论 模拟缺血时Ica.L明显受到抑制,IKATP增加,可能是导致心室肌细胞动作电位时程缩短的主要因素之一。
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| 关 键 词： | 膜片钳术 心肌 心室 离子通道 心肌缺血 |
| 文章编号： | 1008-8296(2003)-02-0084-04 |
Effect of simulated ischemia on ion currents of ventricular myocytes of the guinea pig |
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| Wang Wenwei,Gong Hui,Zhu Yichun,Gong Qianling. Effect of simulated ischemia on ion currents of ventricular myocytes of the guinea pig[J]. Geriatrics & Health Care, 2003, 9(2): 84-86,110 |
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| Authors: | Wang Wenwei Gong Hui Zhu Yichun Gong Qianling |
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| Affiliation: | Wang Wenwei,Gong Hui,Zhu Yichun,Gong Qianling Department of Physiology,Medical Center of Fudan University,Shanghai 200032,China |
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| Abstract: | Objective The effects of simulated ischemia on calcium and potassium ion channels of myocytes were observed. Methods The experiments were carried out on isolated guinea pig ventricular myocytes by using whole-cell patch clamp. Results Whole-cell patch clamp recordings showed that L-type calcium current was inhibited under the condition of simulated ischemia. ATP-sensitive potassium currents were increased under simulated ischemia. Conclusions It is possible that action potential duration shortening is related to the decrease of L-type calcium current and the increase of ATP-sensitive potassium current under simulated ischemia. |
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| Keywords: | Patch clamp techniques Myocardium Heart ventricle Ion channels Myocardial ischemia |
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