柱前衍生化GC法测定旱芹非淀粉多糖中各单糖组分含量

目的建立测定旱芹非淀粉多糖中各单糖组分含量的柱前衍生化GC法。方法非淀粉多糖经衍生化生成糖腈乙酯,采用GC法进行分析。色谱柱:DB-17柱(30 m×0.25 mm,0.25μm);柱温:程序升温;载气:N2;进样温度:270℃;进样方式:不分流;检测器温度:270℃;进样量:1.0μL。结果水不溶性非淀粉多糖(insoluble non-starch polysaccharides,INSP)水解后得到7种单糖,分别是:L-(+)-鼠李糖、L-(+)-阿拉伯糖、D-(+)-岩藻糖、D-(+)-木糖、D-(+)-甘露糖、D-(+)-葡萄糖和D-(+)-半乳糖;水溶性非淀粉多糖(soluble non-starch polysaccharides,SNSP)水解后得到6种单糖,除无D-(+)-岩藻糖外,其余均同INSP水解后单糖。结论本方法准确度高,重现性好,可用于测定旱芹水溶性和水不溶性非淀粉多糖中各单糖组分的含量。

Determination of the contents of monosaccharide components in non-starch polysaccharides of aerial part of Apium graveolens L. by pre-column derivatization GC

Objective To establish pre-column derivatization GC method for determination of monosaccharide component of non-starch polysaccharides of the aerial part Apium graveolens L. Methods The derivatives were determined by GC and DB-17 column(30 m × 0.25 mm, 0.25 μm) was used. The inlet temperature was maintained at 270 °C. The column oven was begun at 180 °C, then programmed from 180 °C to 225 °C at 3 °C·min^-1, hold for 2 min, then programmed from 225 °C to 260 °C at 6 °C·min^-1, and finally, hold for 7 min. Manner of sample injection was splitless. Detector temperature was maintained at 270 °C. Sample amount was 1.0 μL. Results Seven monosaccharides L-(+)-rhamnose, L-(+)-arabinose, D-(+)-fucose, D-(+)-xylose, D-(+)-mannose, D-(+)-glucose and D-(+)-galactose were determined in INSP. Except D-(+)-fucose, other six monosaccharides were the same as from INSP in SNSP. Conclusion The method is accurate and reproducible, which is suitable for the determination of monosaccharide component in solubility and insolubility non-starch polysaccharides in Apium graveolens L.