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| HPLC测定温肾调经颗粒中阿魏酸的含量 | |
| 引用本文： | 闫建坤,李红英,于得才,杨丽. HPLC测定温肾调经颗粒中阿魏酸的含量[J]. 包头医学院学报, 2014, 30(2): 9-10 |
| 作者姓名： | 闫建坤 李红英 于得才 杨丽 |
| 作者单位： | 闫建坤(包头医学院职业技术学院,内蒙古包头,014030)；李红英(山东威高药业股份有限公司)； 于得才 (包头中药有限责任公司)； 杨丽 (包头中药有限责任公司)； |
| 摘 要： | 目的：建立温肾调经颗粒中阿魏酸含量的高效液相色谱测定方法。方法：采用Kromasil C18色谱柱（250×4.6 mm,5μm）,以甲醇-5%醋酸（20∶80）为流动相,流速为1.0 mL/min,检测波长323 nm,柱温35℃。结果：阿魏酸含量测定的线性范围为2.50-20.00μg/mL,r=0.999 9,平均加样回收率为98.54%,RSD为1.22%（n=5）。结论：该方法操作简单,准确可靠,重复性好,可作为温肾调经颗粒的质量控制方法。 |
| 关 键 词： | 温肾调经颗粒 阿魏酸 高效液相色谱 |
Detemination of Ferulic Acid in Wenshentiaojing Granules by HPLC |
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| YAN Jiankun,LI Hongying,YU Decai,YANG Li. Detemination of Ferulic Acid in Wenshentiaojing Granules by HPLC[J]. Journal of Baotou Medical College, 2014, 30(2): 9-10 | |
| Authors: | YAN Jiankun LI Hongying YU Decai YANG Li |
| Affiliation: | 1. Vocational and Technical School, Baotou Medical College, Baotou 014030, China; 2. Shandong Wego Medicine Co. , Ltd. ; 3. Baotou Chinese Pharmaceutical Co. Ltd. ) |
| Abstract: | Objective： To establish the determination of ferulic acid in Wenshentiaojing granules by HPLC. Methods： The chromatographic column was Kromasil C18（ 250 × 4. 6 mm,5 μm） with a temperature of 35 degrees. The mobile phase was methanol-5 % acetic acid（ 20 ∶ 80） with a flow rate of 1. 0 mL / min. The detection wave length was 323 nm. Results： The linear range was between 2. 50 μg / mL and 20. 00 μg / mL,r = 0. 999 9. The average recovery rate was 98. 54 %,RSD was 1. 22 %（ n =5）. Conclusion： The method was simple,accurate,reliable,and reproducible. It could be used in the quality control of Wenshentiaojing granules. |
| Keywords: | Wenshentiaojing granules Ferulic acid HPLC |
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