乳腺纤维腺瘤表面增强拉曼光谱学特点及其临床意义

目的：采用壳层隔绝纳米粒子增强拉曼光谱(SHINRES)检测乳腺纤维腺瘤和正常乳腺组织，通过探讨乳腺纤维腺瘤光谱学特点，探讨乳腺纤维腺瘤的生物学特征和鉴别方法。方法：收集乳腺外科手术患者的乳腺组织冰冻切片，共26例，均为女性，年龄19~59岁，乳腺纤维腺瘤17例，正常乳腺组织9例。冰冻切片解冻后先行普通拉曼光谱检测，加壳层隔绝纳米粒子(SHINs)后再次检测。共收集了243个拉曼光谱和273个SHINRES光谱，所有的光谱均进行基线修正拟合，再将所有的光谱用Adjacent-Averaging算法进行15点平滑。结果：普通拉曼光谱检测，正常乳腺组织特征峰出现在1 090、1 157、1 262、1 300、1 442、1 658、1 745和1 874 cm^-1；
在加入SHINs后，少数特征峰的峰位出现2~3 cm-1位移，其中1 090和1 157 cm^-1相对强度明显增加，出现1 496 cm-1特征峰。纤维腺瘤主要的特征峰出现在751、880、930、1 157、1 262、1 442、1 579、1 658和1 745 cm^-1，其中主导的特征峰应归属为脂类，但可见蛋白酰胺Ⅰ带特征峰。结论：拉曼光谱能够发现乳腺正常组织和纤维腺瘤组织中明显的蛋白酰胺Ⅰ带特征峰的差异。利用SHINs对不同类型的乳腺组织最大增强的特征峰不同以及特征峰的最大增强倍数不同，可
以区分乳腺纤维腺瘤和正常乳腺组织。
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Characteristics of shell-isolated nanoparticle-enhanced Raman spectroscopy of breast fibroadenoma and its clinical significance

Objective To identify the normal breast tissue and breast fibroadenoma tissue by shell-isolated nanoparticle-enhanced Raman spectroscopy(SHINERS),and to explore the biological characteristics of FD and the identification method by discussing its spectroscope characteristics.Methods The frozen section of 26patients(all female,aged 19-59years)were obtained by routine surgical resection.9cases of normal tissue and 17cases of breast fibroadenoma tissue were detected by Raman spectroscopy and then SHINERS technique was utilized.A total of 243Raman and 273SHINERS spectra were obtained.All the spectra were dealt with baseline corrected by fitting and subtracting a third-order polynomial and then smoothed with a 15-point Adjacent-Averaging.Results The characteristic peaks of normal breast tissue appeared in 1 090,1 157,1 262,1 300,1 442,1 658,1 745,and1 874cm-1.After adding SHINs,some peaks shifted in 2-3cm-1,the relative strengths of 1 090and1 157cm-1 were significantly increased,and the 1 496cm-1 characteristic peak appeared.The main characteristic peaks of breast fibroadenoma appeared in 751,880,930,880,1 262,1 442,1 579,1 658,and 1 745cm-1;one of the dominant characteristic peak should belong to lipids,but it can be seen that amideⅠcharacteristic peak of protein became more significant.Conclusion Raman spectra can discover the differences of the characteristic peaks of amide Ⅰ between breast fibroadenoma and normal breast tissues.By virtue of different enhancement effects of SHINs to Raman specific peaks of the various tissues,breast fibroadenoma can be distinguished from normal tissue successfully.