小鼠造血干细胞和P_(388)白血病干细胞对5种抗癌药的敏感性比较

用脾集落形成法比较了5种抗癌药对小鼠造血干细胞(NCFU-S)和P_(388)白血病干细胞(LCFU-S)的作用。三尖杉酯碱、半合成三尖杉酯碱、高三尖杉酯碱和环磷酰胺对两类干细胞作用的剂量—反应曲线呈指数形,效能比依次为5.15,6.01,7.96和9.98。阿糖胞苷对NCFU-S无明显作用,但对LCFU-S杀伤作用强,大剂量时剂量—反应曲线趋于水平。当三尖杉酯碱、高三尖杉酯碱和半合成三尖杉酯碱的剂量分别低于0.30,0.28和0.75mg/kg时,对LCFU-S无明显作用,剂量—反应曲线上出现“肩形”,提示LCFU-S在小剂量三尖杉酯类生物碱作用下,有一受亚致死性损伤后修复过程。

COMPARISON OF THE SENSITIVITY OF MURINE HEMOPOIETIC AND P_(388) LEUKEMIC STEM CELLS TO FIVE ANTITUMOR DRUGS

Effects of five antitumor drugs on the murine normal hemopoietic and P_(388) leukemic colony-forming cells were studied and compared by means of spleen colony assay. Dose-survival curves for the normal spleen colony-forming units (NCFU-S) and the leukemic spleen colony-forming units (LCFU-S) exposed to harringtonine (H), partial synthetic harringtonine (PSH), homoharringtonine (HH), and cyclophosphamide (CP)exhibited exponential forms. The value of efficacy ratios of the four drugs was 5.15, 6.01, 7.96, and 9.98 in the following order for H, PSH, HH, and CP, respectively. All dosage levels of arabinosylcytosine (Ara-C)used showed no distinct effect on the NCFU-S, but exhibited a strong effect on the LCFU-S with increasing dosage; the dose-survival curve for LCFU-S was decreased to a plateau value of 0.2% at the dosage of 500-1000 mg/kg. No obvious effect of H, HH, and PSH on the dose-survival curves was observed when the doses used were less than 0.3, 0.28 and 0.75 mg/kg, respectively.