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Identification of spinothalamic tract cells in fresh, unfixed rat spinal cord |
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| Authors: | L Y Huang S M Carlton W D Willis |
| Affiliation: | Marine Biomedical Institute and Departments of Physiology & Biophysics and Anatomy, University of Texas Medical Branch, Galveston, TX 77550 U.S.A. |
| Abstract: | Diamidino yellow dihydrochloride (DY . 2HCl) can reliably label spinothalamic tract cells both in the perfused, fixed and in the fresh, unfixed rat spinal cord. The major advantages of DY . 2HCl as a retrograde marker are that it leaks from the cells very slowly and can be conveniently identified in unfixed neurons by fluorescence microscopy. DY . 2HCl will be a useful marker for identification of spinothalamic tract cells that are to be studied in vitro. |
| Keywords: | spinothalamic tract cell fresh unfixed spinal cord diamidino yellow dihydrochloride cell identification fluorescent label retrograde marker rat spinal cord |
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