梅毒患者外周血淋巴细胞CD28-B7分子的表达及意义

目的探讨CD28-B7 T淋巴细胞活化协同刺激分子在梅毒发病、发展及转归中的作用。方法应用流式细胞仪和逆转录-聚合酶链反应（RT-PCR）对60例梅毒患者（早期梅毒患者和血清固定患者各30例）和30例正常人外周血淋巴细胞中B7（CD80/CD86）及CD4＋T淋巴细胞中CD28的表达进行检测。结果①梅毒患者外周血淋巴细胞表面CD28的表达水平低于正常对照组,差异有统计学意义（P〈0.05）;血清固定组和早期梅毒组比较,CD28表达水平的差异无统计学意义（P〉0.05）。②梅毒患者外周血淋巴细胞CD80及CD86的表达水平明显低于正常对照组（P〈0.05）,但CD80和CD86的表达水平在血清固定组和早期梅毒组间无统计学意义（P〉0.05）。结论本研究提示梅毒患者外周血淋巴细胞表面CD80、CD86及其受体CD28的表达下调,通过影响CD28-B7协同刺激通路,抑制T细胞尤其是CD4＋T淋巴细胞活性功能,可能参与了梅毒的发病、发展及转归过程。

Expression and significance of CD28-B7 in peripheral blood lymphocytes of patients with syphilis

Objective To investigate the significance of CD28-B7,co-stimulatory molecules of T lymphocytes in the onset,development and prognosis of patients with syphilis.Methods Flow cytometry and RT-PCR technique were applied to detect the expresstion levels of B7(CD80/CD86)in peripheral blood lymphocytes and CD28 in CD4+T lymphocytes from 60 syphilis patients(30 seroresistant syphilis patients and 30 early syphilis patients)and 30 healthy volunteers as control group.Results ①The expression of CD28 in patients with syphilis was lower than what in the control group(P<0.05),but the CD28 level in patients with seroresistant syphilis had no difference from that in patients with early syphilis(P>0.05).②The expression of CD80 and CD86 in patients with syphilis was significantly lower than that in the control group(P<0.05),but the CD80 and CD86 level in patients with seroresistant syphilis had no difference from that in patients with early syphilis(P>0.05).Conclusion The expression of CD80,CD86 and CD28 on peripheral blood lymphocytes in patients with syphilis decreased,which impacted the CD28-B7 co-stimulatory pathway and then the immune function of T cells were impaired.This may be involved in the onset,development and prognosis of syphilis.