| 高浓度葡萄糖对培养兔角膜上皮细胞增殖活性的影响 |
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| 引用本文： | 宋子宣,陈剑,王彦平,阮余霞,陈建苏,吴静,唐光霞,高松哲.高浓度葡萄糖对培养兔角膜上皮细胞增殖活性的影响[J].广东医学,2011,32(10):1224-1226. |
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| 作者姓名： | 宋子宣 陈剑 王彦平 阮余霞 陈建苏 吴静 唐光霞 高松哲 |
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| 作者单位： | 宋子宣,陈剑,阮余霞,高松哲,SONG Zi-xuan,CHEN Jian,RUAN Yu-xia,GAO Song-zhe(暨南大学附属第一医院眼科,广州,510630);王彦平,WANG Yan-ping(暨南大学医学院病理生理学教研室,广州,510632);陈建苏,吴静,唐光霞,CHEN Jian-shu,WU Jing,TANG Guang-xia(暨南大学医学院眼科研究室,广州,510632) |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的 研究高浓度葡萄糖对体外培养兔角膜上皮细胞增殖活性的影响,建立糖尿病性兔角膜上皮细胞培养模型.方法 按照析因设计方法将葡萄糖浓度(25、40、60 mmol/L)和培养时间(24、48、72 h)进行全面组合,应用SunBioTM Am-Blue细胞增殖与活性检测试剂检测兔角膜上皮细胞增殖活性,倒置显微镜观察细胞形...
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| 关 键 词： | 高浓度葡萄糖 角膜上皮细胞 增殖活性 兔 |
Effects of high glucose on the proliferation activity of cultured rabbit corneal epithelial cells |
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| SONG Zi-xuan,CHEN Jian,WANG Yan-ping,RUAN Yu-xia,CHEN Jian-shu,WU Jing,TANG Guang-xia,GAO Song-zhe.Effects of high glucose on the proliferation activity of cultured rabbit corneal epithelial cells[J].Guangdong Medical Journal,2011,32(10):1224-1226. |
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| Authors: | SONG Zi-xuan CHEN Jian WANG Yan-ping RUAN Yu-xia CHEN Jian-shu WU Jing TANG Guang-xia GAO Song-zhe |
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| Institution: | SONG Zi-xuan,CHEN Jian,WANG Yan-ping,RUAN Yu-xia,CHEN Jian-shu,WU Jing,TANG Guang-xia,GAO Song-zhe.The Department of Ophthalmology,the First Affiliated Hospital of Jinan University,Guangzhou 516030,China |
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| Abstract: | Objective To study the effects of high glucose on proliferation activity of cultured rabbit corneal epithelial cells in vitro,and to establish culture model of diabetic rabbit corneal epithelial cells.Methods Rabbit corneal epithelia cells were cultured in glucose concentrations at 25,40 and 60 mmol/L,for 24,48 and 72 hours apiece according to factorial design.SunBioTM Am-Blue cells proliferation and activity assay kit was applied to detect proliferation activity of cultured rabbit corneal epithelial cell i... |
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| Keywords: | high glucose corneal epithelial cells proliferation activity rabbit |
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