达那唑体外对子宫内膜异位症患者腹腔巨噬细胞释放细胞因子 …

目的 了解达那唑体外对子宫内膜异位症（内异症）患者腹腔巨噬细胞释放细胞因子的影响及与胞浆游离钙（「Ｃａ＾２＋」）浓度的关系。方法 将浓度分别为１０μｇ／Ｌ、１００μｇ／Ｌ、１０００μｇ／Ｌ的达那唑加入１４例内异症不孕患者和１１例盆腔正常的不孕患者（对照）的腹腔液中进行培养,用双抗体夹心法检测腹腔巨噬细胞培养上清液中肿瘤坏死因子α（ＴＮＦ－α）和白细胞介素６（ＩＬ－６）的水平。同时,应用Ｆｒｕａ２测

In vitro effect of danazol on cytokine production of macrophages in peritoneal fluid of infertile patients with endometriosis and its relationship with cytosolic free calicum concentration]

OBJECTIVE: To investigate in vitro effect of danazol on cytokine production of macrophages in peritoneal fluid from infertile patients with endometriosis and its relationship with cytosolic free calicum ([Ca2+]i) concentration. METHODS: Pertitoneal macrophages were obtaind from 14 cases of 24 infertile women with endometriosis (EM group) and 11 cases of 19 infertile women with normal pelvis (control group). Danazol was added to peritoneal macrophages culture reaching final concentrrations of 10 micrograms/L, 100 micrograms/L, 1,000 micrograms/L respectively. Tumor necrosis factor alpha(TNF-alpha) and interleukin-6 (IL-6) levels in macrophage supernatant were detected by enzyme linked immunoassay. The [Ca2+]i of cultured peritoneal macrophage and its changes after danazol addition were also studied. RESULTS: In EM group TNF-alpha and IL-6 levels in peritoneal macrophage supernatant and [Ca2+]i of macrophages were elevated significantly as compared with those in control group. When danazol was added to the cell culture and reached 100 micrograms/L of concentration, the levels of TNF-alpha and IL-6 in cultured macrophage supernatant and the [Ca2+]i of macrophages in EM group decreased significantly (P < 0.01). CONCLUSION: It is suggested that danazol may exert its suppressive effect on endometriosis by inhibiting cytokine release of peritoneal macrophages and may related to decline of [Ca2+]i concentration.