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| HPLC法测定夏桑菊颗粒中蒙花苷的含量 | |
| 引用本文： | 高超. HPLC法测定夏桑菊颗粒中蒙花苷的含量[J]. 中国当代医药, 2013, 0(3): 64-65 |
| 作者姓名： | 高超 |
| 作者单位： | 山西医科大学 |
| 摘 要： | 【摘要】目的研究高效液相色谱（HPLC）法测定夏桑菊颗粒中蒙花苷含量的方法。方法采用AgilentZORBAXSB-C18（250mm×4．6mm，5μm）色谱柱，十八烷基硅烷键合硅胶为填充剂；乙腈-0．1％磷酸溶液（70：30）为流动相；检测波长为334nm；流速：1．0mL／min；柱温：30℃。结果蒙花苷含量在0．0476-0．9520μg范围内具有良好线性关系，r=0．9999，平均加样回收率为99．73％，RSD=0．76％（n=6）。结论以HPLC法测定夏桑菊颗粒中蒙花苷的含量，方法简便快捷，精密度高，重现性好，可用于药品质量的控制。 |
| 关 键 词： | 蒙花苷 夏桑菊颗粒 高效液相色谱 含量 |
Determination of Linarin in Xiasangju Granule by HPLC |
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| GAO Chao. Determination of Linarin in Xiasangju Granule by HPLC[J]. http://www.botanicus.org/, 2013, 0(3): 64-65 | |
| Authors: | GAO Chao |
| Affiliation: | GAO Chao Shanxi Medical University, Taiyuan 030001, China |
| Abstract: | Objective To study the method for the determination of Linarin in Xiasangju Granule by HPLC. Methods The Agilent ZORBAX SB-C18 （250 minx4.6 ram,5 txm） was used, octadecylsilane chemically bonded silica was the filler; the mobile phase was acetonitrile-0.1% phosphoric acid （70：30）; the detection wavelength was 334 nm; the flow rate was 1.0 mL/min; the column temperature was 30℃. Results The calibration curve was linear over the ranges of 0.047 6-0.952 0μg （r = 0.999 9） for linarin, the plus sample recovery rate of liuarin was 99.73%, RSD = 0.76% （n = 6）. Conclusion The results shows that the convenient and fast, accurate and reproducible in determination of Linarin con- tent in Xiasangju Granule by HPLC. It can be used for the drug control. |
| Keywords: | Linarin Xiasangju granule High performance liquid chromatography Content |
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