高糖摄入对大鼠糖和脂质代谢及纤溶活性影响

①目的　观察高糖摄入对正常 Ｓｐｒａｇｕｅ Ｄａｗ ｌｅｙ（ Ｓ Ｄ）大鼠糖及胰岛素代谢、脂质代谢和纤溶活性的影响。②方法　将雄性 Ｓ Ｄ 大鼠１６ 只随机分为２ 组，实验组于饮水中添加蔗糖（１２０ｇ／ Ｌ）喂养４２ｄ，对照组给以正常饮水，每７ｄ 定时测量体质量（ Ｂ Ｗ）１ 次，实验最后１ｄ 取血测空腹血糖（ Ｆ Ｂ Ｇ），空腹血清胰岛素（ Ｉ Ｎ Ｓ），甘油三酯（ Ｔ Ｇ），总胆固醇（ Ｔ Ｃ），高密度脂蛋白胆固醇（ Ｈ Ｄ Ｌ Ｃ）水平及血浆纤溶酶原激活物抑制物１（ Ｐ Ａ Ｉ１）活性等指标。③结果与对照组相比，实验组 Ｆ Ｂ Ｇ，血清 Ｔ Ｇ 水平及血浆 Ｐ Ａ Ｉ１ 活性均升高（ｔ＝ ２．３６～４．７３， Ｐ＜ ０．０５，０．０１）；血清 Ｔ Ｃ及 Ｈ Ｄ Ｌ Ｃ水平两组差异无显著性（ｔ＝ １．３７，０．８４， Ｐ＞ ０．０５）；两组血清 Ｉ Ｎ Ｓ水平虽然差异无显著性（ｔ＝ ０．７７， Ｐ＞０．０５），但是经协方差分析纠正体质量因素后，实验组与对照组相比存在高胰岛素血症（ Ｆ＝ ５．７４， Ｐ＜ ０．０５）；直线相关分析提示，实验组血清 Ｉ Ｎ Ｓ水平与 Ｂ Ｗ ，血清 Ｔ Ｇ水平及血浆 Ｐ Ａ Ｉ１ 活性呈高度正相关，对照组血清 Ｉ Ｎ Ｓ水平与 Ｂ Ｗ 及

THE EFFECTS OF HIGH SUCROSE INGESTION ON GLUCOSE AND LIPID METABOLISM AND FIBRINOLYTIC ACTIVITY IN RATS

Objective\ To investigate the effects of high sucrose ingestion on glucose/insulin metabolism, lipid metabolism and fibrinolytic activity in Sprague Dawley(SD) rats.\ Methods\ Sixteen male SD rats were randomly divided into two groups: control group (C, n =8) and sucrose group (S, n =8). The rats in the S group were given sucrose (120g/L) dissolved in tap water as drinking water for 42 days, while the rats in the C group were given tap water. Measurement of body weight(BW) was conducted every seven days. The fast blood glucose(FBG),serum insulin(INS),triglyceride(TG), total cholesterol(TC), high density lipoprotein C (HDL C) levels and plasm plasminogen activator inhibitor type 1(PAI 1) activity were measured on the last day of the experiment.\ Results\ Compared with C group, S group exhibited hyperglycermia, hypertriglceridemia and elevated PAI 1 activity ( t=2.36-4.73,P <0.05,0.01). The levels of TC and HDL C in both groups showed no difference ( t=1.37,0.84,P >0.05). Although the INS levels in both groups had no obvious difference ( t= 0.77 ,P >0.05), when the factors of BWs were corrected by using analysis of covariance, S group exhibited hyperinsulinemia compared with C group ( F=5.74,P <0.05). The INS showed positive correlation with BW, TG and PAI 1 in the S group and with BW and TG in the C group ( r=0.727- 0.915 , P <0.05,0.01).\ Conclusion\ The development of perturbations in glucose/insulin metabolism such as insulin resistance and/or hyperinsulinemia associated with hypertriglyceridemia may play an important role in sucrose induced decreased fibrinolytic activity in SD rats.