| 三种甲替溶解剂在MTT比色法中的应用 |
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| 引用本文： | 郝京生,贾东华,董玉环.三种甲替溶解剂在MTT比色法中的应用[J].河北医科大学学报,1995(6). |
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| 作者姓名： | 郝京生 贾东华 董玉环 |
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| 作者单位： | 北京军区军事医学研究所 |
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| 摘 要： | 研究ＭＴＴ比色法中三种甲 溶解剂，异丙醇（ＩＳＯＰ）、１０％曲拉通—５０％异丙醇（ＴＲＩ－ＴＯＮ－ＩＳＯＰ）和２０％ＳＤＳ（均加入１０ｍｍｏｌ／ＬＨＣｌ酸化）的应用效果。结果发现，在测定不同浓度的外周血单个核细胞（ＰＢＭＣ）时，ＴＲＩＴＯＮ—ＩＳＯＰ与ＳＤＳ效果相当，而在测定不同浓度的Ｈｅｌａ细胞时，前者优于后者，而ＴＲＩＴＯＮ—ＩＳＯＰ和ＳＤＳ均优于ＩＳＯＰ。
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| 关 键 词： | MTT比色法 异丙醇 曲拉通 SDS |
APPLICATION OF THREE SOLUBILIERS OF FORMAZAN IN MTT COLORIMETRIC ASSAY |
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| Hao Jingsheng,Jia Donghua,Dong Yuhuan.APPLICATION OF THREE SOLUBILIERS OF FORMAZAN IN MTT COLORIMETRIC ASSAY[J].Journal of Hebei Medical University,1995(6). |
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| Authors: | Hao Jingsheng Jia Donghua Dong Yuhuan |
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| Institution: | Military Medicine Institute of Beijng Military Region |
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| Abstract: | The effects of three solubilizers of formazan which were iospropanol (ISOP), 10% triton-50% iospropanol (TRITON-ISOP) and 20% sodium dodecyl sulphate (SDS) (all with 10mmol/L HCL) in MTT assay were studied. The results showed the effects of TRITON-ISOP and SDS were similar in determining the concentration of PBMC. TRITON-ISOP was better than SDS, TRITON-ISOP and SDS were superior to ISOP in determining the concentration of Hela cells. |
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| Keywords: | MTT colorimetric assay iospropanol triton SDS |
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