Simultaneous Quantitative Analysis of Five Components in Angelica sinensis and Angelica acutiloba Acclimatized Growing in Vietnam by High-Performance Liquid Chromatography with Photodiode Array Detector |
| |
Authors: | Thi Minh Tam Pham Thi Thu Cuc Le Van Thanh Nguyen Viet Hung Tran |
| |
Institution: | a.Department of Microbiology, Institute of Drug Quality Control, Ho Chi Minh City;a.Department of Microbiology, Institute of Drug Quality Control, Ho Chi Minh City;b.Department of Instrumental Analysis, Institute of Drug Quality Control, Ho Chi Minh City;b.Department of Instrumental Analysis, Institute of Drug Quality Control, Ho Chi Minh City;c.Institute of Drug Quality Control, Ho Chi Minh City; a.Department of Microbiology, Institute of Drug Quality Control, Ho Chi Minh City;d.Faculty of Pharmacy, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam |
| |
Abstract: | Background: Chinese Danggui (Angelica sinensis) and Japanese Danggui (Angelica acutiloba) are evaluated as the same in using and critical
of quality control in Vietnamese Pharmacopoeia. In Vietnam, Japanese Danggui were acclimatized and cultivated in several regions in recent
years. Despite the huge climatic difference between Vietnam and Japan, Japanese Danggui grows very well in Vietnam. However, there are
no studies to assess the overall quality of this medicinal herbs. Aims and Objectives: The aim of this study is to develop a method to identify
and assay simultaneously 5 component compounds of these crude herbs: chlorogenic acid, ferulic acid, scopoletin, xanthotoxin and ligustilide
by high-performance liquid chromatography with photodiode array detector (HPLC-PDA). Materials and Methods: The procedure was
optimized by using column Phenomenex Gemini 5 µm PR C18, 15 x 4,6 mm with the detection wavelength set at 321 nm for detector PDA
and mobile phase was composed of (A) ACN and (B) aqueous solution 1% of acid acetic using a gradient elution. Analytes were performed
at 30°C with a flow rate of 1.0 mL/min. Results: The developped method were fully validated for linearity, accuracy, recovery and met the
validation requirements, proved practical applicability for the quality control of these herbs and related products. Conclusion: The method
was successfully applied to the quantification of five markers simultaneously from collected samples. |
| |
Keywords: | Chlorogenic acid Danggui ferulic acid ligustilide scopoletin xanthotoxin |
本文献已被 CNKI 等数据库收录! |
| 点击此处可从《》浏览原始摘要信息 |
| 点击此处可从《》下载免费的PDF全文 |
|