金果饮的质量控制研究

目的：对金果饮的质量标准进行制定。方法：对玄参、西青果、陈皮建立薄层色谱的鉴别方法；采用高效液相色谱法测定橙皮苷的含量。用Agilent SB C18柱（4.6mm×250mm，5μm），以甲醇-6%乙酸溶液（35∶65）为流动相；检测波长为283nm，流速1mL·min-1。结果：薄层色谱鉴别中，供试品在与对照药材色谱相应的位置上，显示相同颜色的斑点，斑点清晰，分离效果好，阴性无干扰；含量测定法中，橙皮苷在36.35～726.95ng（r=0.9999，n=5）范围内呈良好的线性关系，平均加样回收率为98.58%（RSD为1.85%）。结论：建立的方法简便、准确、专属性强、重复性好，能有效用于金果饮的质量控制。

Study on Quality Control of Jinguo Yin

Objective:To establish quality control of Jinguo Yin.Methods:TLC method was used to differentiate scrophulariae radix, terminalia chebula retz.and citri pericarpium reticulatae. HPLC method was used to determine content of hesperidin.C18 column (4.6mm× 250mm,5μm)was used with methanol and 6%acetic acid(35∶65) as a mobile phase.The flow rate was 1mL·min-1,detection wavelength was 283 nm. Results:Samples and controls had the same color fluorescence spots at the same position in the TLC method.Spots were well isolated.In the HPLC method, linear range was 36.35~726.95ng (r =0.9999, n=5).Average recovery was 98.58%(RSD=1.85%,n=9). Conclusion:The established method was sensitive,accurate,repeatable and specific.It can be used for quality control of Jinguo Yin.