慢性胰腺炎大鼠胰腺K_(ATP)通道表达及药物干预研究

目的 观察慢性胰腺炎大鼠胰腺K_(ATP)通道的Kir6.2和SUR1亚基表达变化,以及那格列奈对其干预作用.方法 Wistar大鼠建立慢性胰腺炎模型后,随机分模型组、给药组和对照组,进行葡萄糖糖耐量实验,RT-PCR法检测各组Kir6.2和SUR1的mRNA水平,Western印迹法检测二者的蛋白表达.结果 模型组大鼠的糖耐量异常,胰腺K_(ATP)通道Kir6.2和SUR1亚基的mRNA水平和蛋白表达显著降低(P＜0.05),给药组对二者的表达显示了一定的上调作用.结论 慢性胰腺炎大鼠胰腺K_(ATP)通道的表达降低,可能是胰源性糖尿病发生的一个重要机制;那格列奈对其表达有一定的上调作用,对于改善慢性胰腺炎病人的预后具有潜在应用价值.

Expression of pancreatic ATP-sensitive K~+ channels in rats with chronic pancreatitis and drug interventions

Objective To observe the expression of pancreatic K_(ATP) channels (Kir6.2/SUR1) in rats with chronic pancreatitis and explore the intervention of nateglinide on the changes.Methods Wistar rats were induced to suffer from chronic pancreatitis and then randomized into model group, nateglinide group and control group.Then OGTT of them were observed.RT-PCR was used to detect the expression of Kir6.2 and SUR1 mRNA, and western blot to detect the expression of Kir6.2 and SUR1 proteins.Results Model rats displayed impaired glucose tolerance (IGT).The expression of Kir6.2 and SUR1 in model group decreased significantly(P＜0.05), and nateglinide displayed up-reg-ulation to the expression in some degree.Conclusion The expression of pancreatic K_(ATP) channels in rats with chronic pancreatitis diminished, which might be the important mechanism of the development of pancreatogenic diabetes.Nateglinide can up-regulate the expression in some degree, which indicates that it may have latent effect of ameliorating the prognosis of patients with chronic pancreatitis.