补康灵对辐射损伤小鼠造血功能的影响

目的:探讨补康灵对辐射损伤小鼠造血功能的影响。方法:50只小鼠被随机分为5组:正常对照组、辐射对照组、补康灵低、中、高剂量组。实验组小鼠在60Coγ射线4 Gy照射后,补康灵组分别灌胃9,18,36 g.kg-1,其余2组给予生理盐水,连续给药10 d,照射后第5,10天检测血象。10 d后处死小鼠,观察补康灵对骨髓DNA含量、骨髓有核细胞数、脾结节的影响。结果:60Coγ射线照射后小鼠外周白细胞、血小板数减少,补康灵给药后,中、高剂量组小鼠外周白细胞、血小板数升高,中、高剂量组骨髓DNA含量吸光度(A)为(1.05±0.27),(1.22±0.26);骨髓有核细胞数为(5.12±1.22),(6.01±1.35)×106/L,脾结节(7.67±1.56),(8.52±2.12)个,均比辐射对照组明显增加(P<0.05)。结论:补康灵照射后给药能促进辐射损伤小鼠造血功能的恢复。

Effects of Bukangling on Hematopoietic Function in Radiation-injured Mice

Objective:To study the effects of Bukangling on hematopoietic function in radiation-injured mice.Method: Fifty mice were randomly divided into five groups:control group,radiation injure group,high,medium and low dose group of Bukangling.9,18,36 g·kg-1 of Bukangling were ig given for 10 days after they were radiated by60Co γ.The others were given normal saline.Peripheral blood routine was measured at the 5th and 10th day after radiation.DNA content,quantity of nucleocyte in bone marrow and(CFUs) in spleen were observed at the 11th day after radiation.Result: The number of peripheral blood routine was lower after radiation,but was higher after administration of Bukangling in medium and high doses group.DNA content was(1.05±0.27),(1.22±0.26)A,the quantity of nucleocyte in bone marrow was(5.12±1.22),(6.01±1.35)×106/L.The number of spleen nodules was(7.67±1.56),(8.52±2.12),they significantly increased in the midium and high doses compared with the radiation group(P<0.01).Conclusion: Bukangling can promote the recovery of the blood cells in bone marrow in radiation-injured mice.