芦荟及其复方提取物对RAW264.7细胞炎症因子的影响

目的:观察芦荟及其复方提取物对小鼠巨噬细胞炎症因子的影响.方法:RAW264.7细胞按2×105/mL稀释,100μL/孔接种入96孔板,药物质量浓度为250,125,62.5 g?L-1下采用噻唑蓝比色法(MTT法)作用4h,检测芦荟及其复方提取物对小鼠巨噬细胞的毒性作用;药物质量浓度为50,25,12.5 g?L-1下采用硝酸还原酶法作用24 h,测定小鼠巨噬细胞一氧化氮(N0)含量,并用酶联免疫吸附法(ELISA法)测定其在单纯药物及脂多糖(LPS)1 mg?L-1诱导情况下小鼠巨噬细胞肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10)的含量.结果:芦荟及其复方提取物具有促进巨噬细胞释放NO的作用,并与药物浓度成正相关(P＜0.01);芦荟有促进TNF-α分泌(P＜0.01)和抑制IL-10分泌(P＜0.05)的作用,其复方在LPS诱导下则对TNF-α和IL-10都有抑制作用(P＜0.01).结论:芦荟在250～7.8 g?L-1下对臣噬细胞无细胞毒性,其复方则有一定毒性;芦荟及其复方对小鼠巨噬细胞炎症因子的释放有一定影响.

Effects of Aloe vera and Compound Extract of Aloe vera on Inflammatory Factors in RAW264.7 Cells

Objective:To observe effect of the Aloe vera and its compound extract on inflammatory factors.Method: Cells diluted by 2×105/mL were inoculated into 96-well plates,100 μL per well,concentrations of druds was 250,125,62.5 g·L-1,MTT method was assay to test the toxic effects of macrophages.When the concentrations was 50,25,12.5 g·L-1 macrophage nitric oxide(NO) levels was measured.Enzyme-linked immunosorbent assay(ELISA) were used to measure tumor necrosis factor-α(TNF-α) and interleukin-10(IL-10) levels in pure drug and lipopolysaccharide(LPS)-induced murine macrophage.Result:Aloe vera and its compound extract promoted releasing of NO,with a concentration dependent manner(P<0.01);Aloe vera could promote TNF-α secretion(P<0.01) and inhibition of IL-10 secretion(P<0.05),the compound inhibited LPS induced releasing of TNF-α and IL-10(P<0.01).Conclusion: Aloe in 250-7.8 g·L-1 concentration has non-cytotoxic macrophages,compound extract has a certain toxic action,and can exert some influence on release of inflammatory factors.