MAGE-A1在食管癌中的表达及其与肿瘤细胞增殖的关系

目的:研究食管癌中肿瘤特异性抗原MAGE-A1,Ki-67的表达及其相关性,探讨他们与食管癌生物学行为及生存时间的关系.方法:免疫组化SP法,利用鼠抗人MAGE-A1,Ki-67单克隆抗体检测60例食管癌标本中MAGE-A1,Ki-67表达水平.结果:60例食管癌中MAGE-A1表达率为83.3%(50/60),高表达率为10%(6160),低表达率为73.3%(44/60),正常组织表达率为0%,食管癌和正常组织之间的表达具有显著性差异(P＜0.001).Ki-67表达率为93.3%(56/60),高表达率为70%(42/60),低表达率为23.3%(14/60),正常组织表达率为13.3%(4/30),食管癌和正常组织之间的表达具有显著性差异(P＜0.001).MAGE-A1与分化程度有关(P＜0.05),Ki-67与年龄、分化程度、病理类型有关(P＜0.05).MAGE-A1阳性表达的高低与Ki-67阳性表达的高低呈负相关(r=-2.91,P＜0.05).MAGE-A1阳性患者的生存时间明显高于阴性者.结论:MAGE-A1在食管癌中有较高的表达,并且与细胞增殖相关,正常组织不表达,MAGE-A1阳性患者的预后好,有望作为免疫治疗的靶点.

Expression of melanoma antigen A1 and its relationship with tumor cell proliferation in esophageal carcinoma

AIM: To investigate the expression of melanoma antigen A1 (MAGE-A1) and Ki-67 in esophageal cancer (EC) and their correlations with the clinical pathological features and survival time of EC patients. METHODS: The expression of MAGE-A1 and Ki-67 was detected by SP immunohistochemical technique using mouse anti-human MAGE-A1 and Ki-67 antibodies in formalin-fixed and par-affin-embedded sections from 60 EC patients. RESULTS: The positive rate of MAGE-A1 ex-pression was 83.3% (50/60) in EC,with a high expression rate of 10% (6/60) and low expres-sion rate of 73.3% (44/60),while the positive rate of MAGE-A1 expression was 0% in the normal esophageal tissues,significantly lower than that in EC tissues (P < 0.001). The positive rate of Ki-67 expression was 93.3% (56/60) in EC,with a high expression rate of 70% (42/60) and low expression rate of 23.3% (14/60),while the posi-tive rate of Ki-67 expression was 13.3% (4/30) in esophageal normal tissues,significantly lower than that in EC tissues (P < 0.001). There was a negative correlations between the positive ex-pression of MAGE-A1 and Ki-67 (r=-2.91,P < 0.05). Besides,MAGE-A1 expression was also correlated with the differentiation degrees of car-cinoma (P < 0.05),and Ki-67 expression was cor-related with the age of patients,differentiation degrees and pathological type of carcinoma (P < 0.05). The survival time was higher for patients with the positive expression of MAGE-A1 than that for patients with the negative MAGE-A1 ex-pression (P < 0.05). CONCLUSION: MAGE-A1 is highly expressed in EC patients,which is correlated with the proliferation of tumor cells. The patients with positive MAGE-A1 expression have a favorable prognosis.