| 同种CTL识别表位的抗原肽非依赖性现象 |
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| 引用本文： | 蔡蕾,翁秀芳,梁智辉,吴雄文.同种CTL识别表位的抗原肽非依赖性现象[J].华中科技大学学报(医学版),2006,35(6):822-824. |
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| 作者姓名： | 蔡蕾 翁秀芳 梁智辉 吴雄文 |
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| 作者单位： | 1. 华中科技大学同济医学院基础医学院细胞生物学系,武汉,430030
2. 华中科技大学同济医学院基础医学院免疫学系,武汉,430030 |
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| 摘 要： | 目的 初步探讨细胞毒T细胞(CTL)对同种抗原的识别机制.方法 用负载特定肽的T2细胞与HLA-A2阴性个体的外周血淋巴细胞(peripheral blood lymphocytes,PBL)共培养,激活同种反应性CTL,通过乳酸脱氢酶(LDH)释放法观察同种反应性CTL对不同载肽T2细胞的杀伤活性.结果 用负载特定抗原肽的T2细胞诱导所获得的同种反应性CTL对负载不同抗原肽的T2细胞及空载T2细胞具有同等杀伤效应.结论 同种反应性CTL对同种MHC分子的识别不依赖抗原肽.
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| 关 键 词： | 同种反应性细胞毒T细胞 HLA-A2 抗原肽 表位 |
| 修稿时间： | 2006年4月19日 |
Antigenic Peptide-independent Phenomena of the Epitope Recognized by Allogeneic CTL |
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| Cai Lei.Antigenic Peptide-independent Phenomena of the Epitope Recognized by Allogeneic CTL[J].Journal of Huazhong University of Science and Technology(Health Sciences),2006,35(6):822-824. |
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| Authors: | Cai Lei |
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| Abstract: | Objective To investigate the mechanism of allogeneic recognition.Methods Peptides loaded T2 cells were used as stimulators to stimulate the HLA-A2~-donors peripheral blood lymphocytes(PBL) proliferating into alloreactive cytotoxic T lymphocytes(CTL).The alloreactive CTLs were used to test cytotoxicity with different targets(T2 cells with different peptides loaded).Results Alloreactive CTLs induced by the either 2 kinds of peptides loaded T2 had cytotoxicity targeting both peptides loaded T2 and null T2 with similar magnitude.Conclusion Allogeneic recognition of alloreactive CTLs to MHC molecules was antigenic peptide-independent. |
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| Keywords: | HLA-A2 |
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