| 人近端肾小管上皮细胞黏附分子CD146表达对细胞凋亡的影响 |
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| 引用本文： | 吴乔木,汪年松,倪培华,王锋,范瑛,吴恒兰. 人近端肾小管上皮细胞黏附分子CD146表达对细胞凋亡的影响[J]. 中国中西医结合肾病杂志, 2009, 10(5): 396-399 |
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| 作者姓名： | 吴乔木 汪年松 倪培华 王锋 范瑛 吴恒兰 |
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| 作者单位： | 1. 上海交通大学医学院附属第六人民医院肾内科,上海,200233
2. 上海交通大学医学院附属瑞金医院检验科,上海,200020 |
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| 摘 要： | 目的：探讨人近端肾小管上皮细胞（HK-2）黏附分子CD146表达与细胞凋亡的关系。方法：体外培养的HK-2N胞在不同浓度葡萄糖和不同浓度甘露醇刺激下分别作用24h、48h、72h,经流式细胞仪（FCM）检测各组HK-2细胞CD146的表达和凋亡率的变化。结果：正常糖浓度下HK2微弱表达CD146,且延长作用时间对表达无影响;随糖浓度升高,CD146表达上调,细胞凋亡率也增高,延长刺激时间可促进CD146表达和细胞凋亡（P〈0．05）;当糖浓度达到40mmol／L,刺激时间达到48h,CD,46表达显著升高（P〈0．05）,HK-2细胞凋亡率也显著上升（P〈0．05）;甘露醇对HK-2细胞CD146表达和细胞凋亡的促进作用较葡萄糖的作用显著降低（P〈0．01）。结论：HK-2组成性表达CD146,高糖诱导CD146表达,甘露醇诱导作用较高糖弱;高糖诱导HK-2凋亡,其凋亡作用与CD146表达增高联系密切,提示黏附分子CD146对细胞生存有重要影响。
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| 关 键 词： | 黏附分子 葡萄糖 甘露醇 人肾小管上皮细胞 |
Effect of CD_(146) Expressed by Human Proximal Renal Tubular Epithelial Cells on Cell Apoptosis |
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| WU Qiaomu,WANG Niansong,NI Peihua,et al. Effect of CD_(146) Expressed by Human Proximal Renal Tubular Epithelial Cells on Cell Apoptosis[J]. Chinese Journal of Integrated Traditional and Western Nephrology, 2009, 10(5): 396-399 |
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| Authors: | WU Qiaomu WANG Niansong NI Peihua et al |
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| Abstract: | Objective:To explore the relationship between CD146 expression and apoptosis of HK-2 cells.Methods:CD146 expression on HK-2 cells and the apoptosis of HK-2 cells under normal and high glucose for 24 h,48 h,72 h were determined by FCM.Results:Weak expression of CD146 in the Normal glucose group(NG5 mM)was found,and its expression exhibited similar level when the stimulation time was extended.As the concentration of glucose was increased,CD146 expression and the apoptosis of HK-2 were up-regulated,both these ... |
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| Keywords: | Adhesive molecule Glucose Mannitol Human renal tubular epithelial cell（HK- 2） |
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