昙花化学成分的研究

 目的 研究昙花的化学成分。方法 采用活性追踪，利用柱色谱和HPLC技术进行分离，根据理化性质和波谱数据进行结构鉴定。结果 昙花甲醇提取物的乙酸乙酯萃取部分显示显著的B-16生长抑制活性，从中分离鉴定了9个化合物，分别为腺苷（1），2′-脱氧腺苷（2），苄基β-D-吡喃葡萄糖苷（3），异鼠李素-3-O-α-L-吡喃鼠李糖(1→6)-［α-L-吡喃鼠李糖(1→2)］-β-D-吡喃半乳糖苷（4），山柰酚-3-O-新橙皮糖苷（5），金丝桃苷（6），山柰酚-3-洋槐糖苷（7），异鼠李素-3-O-β-D-吡喃半乳糖苷（8）和异鼠李素-3-O-洋槐糖苷（9）。结论 所有化合物均为首次从昙花属（Epiphyllum）植物中分离得到，其中化合物2、3、4、5和6为仙人掌科（Cactaceae）植物中首次报道。

Studies on Chemical Constituents of Epiphyllum oxypetalum

OBJECTIVE To investigate the chemical compositions of Epiphyllum oxypetalum (DC.) Haw. METHODS Chemical constituents were isolated by bioassay-guided fractionation, and column chromatography and HPLC. Physico-chemical characters and spectroscopic analyses were employed for the strucrural identification. RESULTS The ethyl acetate partition of methanol extract of E. oxypetalum, which showed strong inhibitory activity against B-16 cells, yielded 9 compounds. They were adenosine (1), 2′-deoxyadenosine (2), benzyl β-D-glucopyranoside (3), isorhamnetin 3-O-α-L-rhamnopyranosyl(1→6)-［α-L-rhamnopyranosyl(1→2)］-β-D-galactopyranoside (4), kaempferol 3-O-neohesperidoside (5), hyperoside (6), kaempferol-3-robinobioside (7), isorhamnetin 3-O-β-D-galactopyranoside (8) and isorhamnetin 3-O-robinobioside (9). CONCLUSION All of these compounds are obtained from this Epiphyllum genus for the first time, and compound 2, 3, 4, 5 and 6 are firstly reported from the Cactaceae family.