体外受精后多原核孕卵生成的影响因素分析

目的：探讨体外受精后多原核孕卵生成的影响因素，为降低临床异常受精率探寻可行方法。方法：应用卡方检验分析927个体外受精周期研究多PN生成率与体外受精方式，女方年龄，超促排卵方案，HCG日血清Ez水平和获卵数的关系。结果：（1）常规IVF周期后多PN生成率显著高于ICSI组；当获卵数〉15个和HCG日血清E。〉4000pg／mt时，体外受精周期中多PN生成率显著升高；（2）ICSI周期中，随着女方年龄的增高，多PN生成率显著增高。结论：体外受精周期中多PN生成的机制和影响因素不尽相同，针对不同不孕因素和年龄等人群选择合适的体外受精方式有助于提高正常受精率。

Analysis of Factors Affecting Polypronuclear in Vitro Fertilization

Objective：To investigate the factors affecting polypronuclear in vitro fertilization. Methods：6815 fertilized ovum of 9718 oocytes from 608 IVF cycles and 319 ICSI cycles were analyzed by analysis of variance to show the influence of the age of women, the stimulating protocol, serum estradiol level on day of hCG , the number of retrieved oocytes on the incidence of polypronuclear. Results： （1） In IVF group, the incidence of polypronuclear was higher than that in ICSI group, but total fertility rate was inferior to the latter. And the incidence of polypronuclear was significantly increased when retrieved oocytes were more than 15 or serum estradiol level on day of hCG was more than 4000 pg/ ml. （2）In ICSI group, the incidence of polypronuclear was significantly increased when the age of female was older than 30 years old. Conclusion： The affecting factors and incidence mechanism are different between IVF and ICSI, making sure that every couple of infertility has optimal modality in vitro fertilization will help to decline the incidence of polypronuclear.