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| 植物细胞膜固定化培养及产物释放行为研究 | |
| 引用本文： | 袁其朋,元英进,胡宗定. 植物细胞膜固定化培养及产物释放行为研究[J]. 中草药, 1996, 27(5): 271-275 |
| 作者姓名： | 袁其朋 元英进 胡宗定 |
| 作者单位： | 天津大学化工系!300072 |
| 摘 要： | 研究了植物细胞膜固定化培养中各种参数对反应器性能的影响，结果表明：细胞层厚度为7～8mm时生产能力可达到较高的水平；孔径为80μm的不锈钢丝网是合适的固定化长春花细胞膜材料；压力脉冲是促进胞内生物碱释放的有效手段；并在理论上建立计算反应器允许细胞层厚度的方法，计算结果与实验吻合较好。 |
| 关 键 词： | 膜反应器 长春花细胞 释放 |
| 收稿时间： | 1995-07-12 |
Study on Immobilization Plant Cell Culture and Release of Intracellular Metabolites |
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| Yuan Qipeng,Yuan Yingjin and Hu Zongding. Study on Immobilization Plant Cell Culture and Release of Intracellular Metabolites[J]. Chinese Traditional and Herbal Drugs, 1996, 27(5): 271-275 | |
| Authors: | Yuan Qipeng Yuan Yingjin Hu Zongding |
| Affiliation: | Department of Chemical Engineering,Tianjin University,Tianjin;Department of Chemical Engineering,Tianjin University,Tianjin;Department of Chemical Engineering,Tianjin University,Tianjin |
| Abstract: | Membrance immobilization of Catharathus roseus(L.)G. Don cell culture was studied.Results showed that the production capacity can attain a hagher level when the cell layer was 7-8mm thick. Stainless-steel. A method to determine allowable cell layer thickness was developed. The calculated calue agreed well with experimental results. |
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